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Ecotoxicological information

Toxicity to microorganisms

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Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Read across study
Justification for type of information:
Read-across justification appended to Section 13 of the IUCLID file.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Maximal soluble amount of test material (saturation concentration) diluted in doubly distilled water. Test concentrations prepared by serial dilution of initial saturated solution.

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida MIGULA, strain Berlin 33/2, from the German Microorganism culture Collection (D-3300 Braunschweig)

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
16 h
Test temperature:
25 ±1 °C
Nominal and measured concentrations:
Nominal: 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L

Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
6 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
0.83 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L, respectively.
Executive summary:

A study was conducted to assess the toxicity of the read across substance, substance amides, C8-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl), to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test material at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 h. Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L, respectively (Poth, 1992).

Description of key information

Key value for chemical safety assessment

EC50 for microorganisms:
6 000 mg/L
EC10 or NOEC for microorganisms:
830 mg/L

Additional information

A study was conducted to assess the toxicity of the read across substance, substance amides, C8-18 (even-numbered) and C18-unsatd., N,N-bis(hydroxyethyl), to the bacteria Pseudomonas putida according to DIN Guideline 38412 L8, in compliance with GLP. Pseudomonas putida colonies, in triplicate, were exposed to the test material at nominal concentrations of 0.01, 0.05, 0.10, 0.50, 1.00, 2.50, 5.00 and 10.00 g/L. Inhibition of cell growth compared to controls was recorded after 72 h. Under the conditions of the study, the EC50 and EC10 were 6.0 and 0.83 g/L, respectively (Poth, 1992).