Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-470-5 | CAS number: 2440-22-4
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
No toxic effects could be observed in a study with Eisenia fetida.
Key value for chemical safety assessment
Additional information
In a 56-day study, adult earthworms of the species Eisenia fetida were exposed to the test substance in concentrations of 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight, in accordance with the OECD 222 guideline. The mortality and the body weight change of the adult worms were observed and recorded on day 28. The reproduction, number of juveniles per test vessel 56 days after application was observed.
Adult worms:
No mortality of adult worms was observed in the controls and at all replicates with
test substance
NOECMortality: ≥ 1000 mg test substance/kg soil (dw)
LOECMortality: > 1000 mg test substance /kg soil (dw).
NOECBiomass change: ≥ 1000 mg test substance/kg soil (dw)
LOECBiomass change: > 1000 mg test substance /kg soil (dw)
Juvenile worms:
Reproduction by count of juvenile worms:
NOECReproduction: ≥ 1000 mg test substance/kg soil (dw)
LOECReproduction: > 1000 mg test substance/kg soil (dw)
No ECx calculations were performed, because there was no dose response relationship. A significant effect was observed at 500 mg/kg of test substances (Wilcoxon test, one sided -; p ≤0.05). However, this can be disregarded as the highest concentration (1000 mg/kg test substance/kg soil (dw)) and a lower concentration than this (125 mg/kg test substance/kg soil (dw)) showed similar number of offspring which is the range of reproduction in control assays. Therefore, we can conclude that this observation is an outlier.
The results in this study are consistent with all relevant validity criteria and the test is valid according to the test guideline of this study. No deviations from the test guidelines or other incidents occurred during the reported test, which may have influenced the results.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
