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Aquatic toxicity studies with ‘benzene, ethylenated, by-products from’ were conducted in fish, invertebrates, algal species, and microorganisms.

For fish, one reliable acute study with rainbow trout and fathead minnow each were available for assessment. The lowest LC50 for fish was >0.97 mg/L and based on the 96 -hour exposure to rainbow trout.

For invertebrates, one reliable acute study with Ceriodaphnia dubia, Daphnia magna, and Chironomous tentans each were available for assessment. The lowest endpoint value for invertebrates was the 48-hour LC50 value of 0.029 mg/L from the test with C. dubia.

For the algal species, one reliable study with Selenastrum capricornutum as the test species was available for assessment. The 72-hour growth rate EC50 (ErC50) and NOEC was 0.485 and 0.0959 mg/L, respectively.

For microorganisms, an activated sludge respiration inhibition assay was available for assessment. The EC50 was >100 mg/L and based on respiration inhibition using a 3 hour contact time.

No long-term toxicity to fish or long-term toxicity to invertebrates was available. The supported uses for this substance are limited to industrial closed systems and as such are not expected to lead to direct or indirect exposure to the environment. Therefore long-term toxicity to fish and long-term toxicity to invertebrates for the substance are not required 

Taken together, the lowest aquatic toxicity value for ‘benzene, ethylenated, by-products from’ was the 48-hour LC50 of 0.029 mg/L, based on the 48-hour acute toxicity test with Ceriodaphnia dubia.