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Short-term toxicity to fish

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Description of key information

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
150 mg/L

Additional information

The acute toxicity of P5524 (which contains 99.9% DEC) to rainbow trout (Oncorhynchus mykiss) was determined in a static GLP compliant test according to EU method C.1 and OECD 203 (Bell 1996). The test was started with a Control (0), 10, 22, 46, 100, and 220 mg test item/L. Dose verification at hour 0 and hour 72 confirmed that the test solutions were correctly dosed (recoveries of the biologically relevant concentrations in general within 80 - 120%. For each test concentration and the control, 1 replicate with 7 fish (mean total length 5.5 cm, average weight 2.16 g/fish) each were set up in 18 L test solution (glass vessels). The test was performed in a 16:8 h light dark cycle. The concentrations were reported as nominal concentrations. After 96 hours of exposure, 0, 0, 0, 0, 0, 0, and 100 % of the daphnids were immobilized at Control, 10, 22, 46, 100, and 220 mg test item/L. The corresponding 96 h LC50 value is 150 mg test item/L.

This study was performed according to international guidelines and under GLP and fulfilled the validity criteria. Correct dosing was verified. Therefore, this study was considered to be Klimisch 1 study.

The obtained results are considered as relevant for the risk assessment.