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EC number: 603-491-5 | CAS number: 131513-00-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The LC50 was after 48 h 2.10 mg/L, after 72 h 1.45 mg/L and after 96 h 1.05 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.05 mg/L
Additional information
Key Study
The acute toxicity of the test item to fish (zebrafish), was determined according to OECD-Guideline for Testing of Chemicals No 203 (1992) and EU Council Regulation No. 440/2008/ C.1 (2008). A semi-static test with daily renewal of the test media was conducted with the nominal test item concentrations of 0.625 - 1.25 - 2.50 - 5.00 - 10.0 mg/L (factor 2), corresponding to mean measured concentrations of 0.251 - 0.621 - 1.03- 2.05 - 5.19 mg/L. Duration of the test was 96 hours. 7 test organisms were exposed to each test concentrations and control. Water quality parameters temperature, pH-value and O2 -saturation measured at 0, 24, 48, 72 and 96 hours were within the acceptable Iimits. The determination of the concentrations of the test item was carried out via HPLC-DAD from freshly prepared media after 0 and 72 h, and from the corresponding 24 h old test media after 24 and 96 h. The measured concentrations in freshly prepared media were in the range of 43 - 76 % of the nominal values and 8 - 46 % in 24 h aged test media. The LC50 was 2.10 mg/L after 48h, 1.45 mg/L after 72h and 1.05 mg/L after 96h (Basedon mean measured test item concentrations). Additionally to the test item another compound with a relative retention time of approx. 0.34 (absorption maximum 291 nm) was detected in all exposure concentrations at test end.
Supporting Study
The purpose of this study was to determine the acute toxicity of the test item on the Zebrafish (Brachydanio rerio). The study procedure was based an the EEC guideline using a semistatic procedure and 100 mg/L Tween 80 as auxiliary compound. To reduce the speed of hydrolysis all test solutions were adjusted to about pH 7 using hydrochlorid acid. Zebrafish were exposed over a 96 -hour period to a range of 7 concentrations spaced by a factor of about 2.2: 0.0464. 0.1, 0.215, 0.464, 1.0, 2.15 and 4.64 mg/L and controls. Since the analytical determinations indicated a loss of the test compound of more than 20% after 48 hours the results were calculated also related to the anaIytically determined values. To study a possible fortification of the acute toxicity by using the auxiliary compound Tween 80, the concentrations 1.0 and 4.64 mg/L were run in parallel without the addition of Tween 80: Tween 80 did not increase the acute toxicity of the test compound in this study.
Results (on the basis of the nominal concentrations):
LC 50 after 96 hours
Greater 1.00 mg/L ( 5% Significance Level)
Smaller: 2.2 mg/L ( 1% Significance Level)
Analytically determined:
LC 50 (96 h)
Greater 0.49 mg/L (5 % Significance Level)
Smaller 1.1 mg/L (1% Significance Level)
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