Phenyl isocyanate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Principles of method if other than guideline:

Five or ten male or female Wister rats were treated semiocclusive with 100, 500, 2500, 3500 or 5000 µl/kg phenyl isocyanate for 24 hours. The animals were observed for mortality, body weight and clinical signs through day 14. A gross pathological examination was performed on animals which died intercurrent or were killed after termination of the study.

GLP compliance:

not specified

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Phenyl isocyanate

EC Number:

203-137-6

EC Name:

Phenyl isocyanate

Cas Number:

103-71-9

Molecular formula:

C7H5NO

IUPAC Name:

isocyanatobenzene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: cellulose powder

Duration of exposure:

24 hours

Doses:

100, 500, 2500, 3500 or 5000 µl/kg = ca. 109.5, 547.5, 2737.5, 3832.5, 5475 mg/kg
density = 1,095 kg/L (20 °C) according LXS MSDS

No. of animals per sex per dose:

5 or 10 male or female animals/dose

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 5 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

Males: 0 (100 µl/kg bw), 0 (500 µl/kg bw), 0 (2500 µl/kg bw), 2 (3500 µl/kg bw), 6 (5000 µl/kg bw)
Females: 0 (100 µl/kg bw), 0 (500 µl/kg bw), 0 (2500 µl/kg bw), 1 (3500 µl/kg bw), 2 (5000 µl/kg bw)

Clinical signs:

other: Immediately sensation of pain, decreased motility, closed eye lids, aggresivity (only males), laboured breathing, weakness of hind limbs, partly strong weight loss. Start of the symptoms: immediately to 15 minutes after application. Duration of the sympto

Gross pathology:

Necropsy findings on animals died intercurrent: lung was inflated and mottled, renal pelvis partly reddened, digestive tract normally without content.
Necropsy findings on animals killed after post-observation period: in the lethal dose range lungs were inflated, other internal organs macroscopically without findings.

Any other information on results incl. tables

Immediately sensation of pain, decreased motility, closed eye lids, aggresivity (only males), laboured breathing, weakness of hind limbs, partly strong weight loss.

Start of the symptoms: immediately to 15 minutes after application. Duration of the symptoms: during the observation period.

Necropsy findings on animals died intercurrent: lung was inflated and mottled, renal pelvis partly reddened, digestive tract normally without content.

Necropsy findings on animals killed after post-observation period: in the lethal dose range lungs were inflated, other internal organs macroscopically without findings.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

LD50 was > 5000 mg/kg bw (rat, male + female). According to CLP classification criteria (Regulation (EC) No 1272/2008) a classification is therefore not justified.

Executive summary:

Five or ten male or female Wister rats were treated semiocclusive with 100, 500, 2500, 3500 or 5000 µl/kg phenyl isocyanate for 24 hours. The animals were observed for mortality, body weight and clinical signs through day 14. A gross pathological examination was performed on animals which died intercurrent or were killed after termination of the study.

LD50 > 5000 mg/kg bw (rat, male + female).