3-chloroaniline

Endpoint summary

Additional information

Short-term toxicity to fish

No. of studies were reviewed for Short-term toxicity to fish from reliable sources having Klimisch rating 2.

The summary of the results are presented below  

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	0.147 mg/L	Mortality	96 hrs.	Danio rerio	Experimental data from Science of the Total Environment for CAS : 108-42-9
2	NOEC	5 mg/L	-	24 hrs.	Petromyzon marinus	Experimental data from Spec.Sci.Rep.Fish.No.207 for CAS : 108-42-9

 

Based on the studies summarized in the above table for the target substance 3-chloroaniline,the endpoint value was found to vary between LC50 = 0.147 mg/L and NOEC = 5 mg/L in a 24 hrs to 96 hrs. study respectively on the basis of mortality effect. Based on the LC50 value and considering the CLP Criteria for aquatic classification of the substance , it is concluded that 3-chloroaniline exhibits short term toxicity to fish (Danio rerio and Petromyzon marinus ) in aquatic Acute Category 1.

Long-term toxicity to fish

The No-observable-effect-concentration (NOEC) value of 3-chloroaniline in the fish [Danio rerio (Zebrafish)] in a 21 days study period on the basis of reproduction effect was found to be 5.6 mg/L.

Short-term toxicity to aquatic invertebrates

No. of studies were reviewed for Short-term toxicity to aquatic invertebrates from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below    

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	EC50	0.35 mg/L	Intoxication	48 hrs.	Daphnia magna (Water Flea)	Experimental data fromWater Researchfor CAS : 108-42-9
2	EC0	0.4 mg/L	Behaviour	24 hrs.	Daphnia magna (Water Flea)	Experimental data from Forschungsbericht for CAS : 108-42-9
3	LC50	0.1 mg/L	Mortality	48 hrs.	Daphnia magna (Water Flea)	Experimental data for CAS : 108-42-9
4	EC50	0.44 mg/L	Intoxication	48 hrs.	Daphnia longispina	Experimental data fromEnvironmentalToxicology and Chemistryfor RA CAS : 95-76-1

 

Based on the studies summarized in the above table for the target substance 3-chloroaniline and the read across substance 3,4-dichloroaniline,the endpoint value was found to vary between effective concentration (EC50) = 0.35 mg/L to 0.44 mg/L,lethal concentration (LC50) = 0.1 mg/L and EC0 = 0.4 mg/L in a 24 hr. to 48 hrs. study on the basis of intoxication,behaviour and mortality effect. Based on the EC50,EC0 and LC50 values and considering the CLP Criteria for aquatic classification of the substance , it is concluded that 3-chloroaniline exhibits short term toxicity to aquatic invertebrate (Daphnia magna and Daphnia longispina) in aquatic Acute Category 1.

Long-term toxicity to aquatic invertebrates

No. of studies were reviewed for Long-term toxicity to aquatic invertebrates from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below  

 

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	NOEC	0.013 mg/L	Reproduction	21 days	Daphnia magna	Experimental data from Water Res for CAS : 108-42-9
2	LC50	0.26 mg/L	Mortality	21 days	Daphnia magna	Experimental data for CAS : 108-42-9

 

Based on the studies summarized in the above table for the target substance 3-chloroaniline,the endpoint value was found to vary between the no observed effect concentration (NOEC) = 0.013 mg/L and lethal concentration (LC50) = 0.26 mg/L in a 21 days study on the basis of mortality and reproduction effect.

 

Toxicity to aquatic algae and cyanobacteria

No. of studies were reviewed for toxicity to aquatic algae and cyanobacteria from reliable sources having Klimisch rating 2 and 4.

The summary of the results are presented below  

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	EC50	21 mg/L	Growth rate	96 hrs.	Chlorella pyrenoidosa	Experimental data  for CAS : 108-42-9
2	EC50	53 mg/L	Population	48 hrs.	Scenedesmus subspicatus (new name: Desmodesmus subspicatus)	Experimental data fromWater Researchfor CAS : 108-42-9
3	EC50	26 mg/L	Population	48 hrs.	Scenedesmus subspicatus (new name: Desmodesmus subspicatus)	Experimental data fromWater Researchfor CAS : 108-42-9
4	EC50	0.74 mg/L	Population	96 hrs.	Pseudokirchnerella subcapitata	Experimental data from study report for RA CAS : 95-76-1

 

Based on the studies summarized in the above table for the target substance 3-chloroaniline and the read across substance,the endpoint value was found to vary between EC50 = 0.74 mg/L to 53 mg/L in a 48 hrs. to 96 hrs. study on the basis of growth rate and population effect. Based on the EC50 values ranging from 21 mg/L to 53 mg/L,the substance qualify for the aquatic classification in Category 3.But EC50 value of read across substance was found to be 0.74 mg/L which exhibits toxicity in aquatic Acute Category 1.

Toxicity to microorganisms

No. of studies were reviewed for toxicity to microorganisms from reliable sources having Klimisch rating 2.

The summary of the results are presented below  

Sr.No	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	IGC50	99 mg/L	Growth inhibition	48 hrs.	Tetrahymena pyriformis	Experimental data from Toxicology methods for CAS : 108-42-9
2	EC50	343 mg/L	Growth inhibition	12 hrs.	Escherichia coli	Experimental data from Ecotoxicology and Environmental Safety for CAS : 108-42-9
3	EC50	100 mg/L	Population	24 hrs.	Tetrahymena pyriformis	Experimental data from Sci. Total Environ.for CAS : 108-42-9

 

Based on the studies summarized in the above table for the target substance 3-chloroaniline in microorganisms (Tetrahymena pyriformis and Escherichia coli),the endpoint value was found to vary between IGC50 = 99 mg/L and EC50 = 100 mg/L to 343 mg/L in a 12 hrs. to 48 hrs. study on the basis of growth inhibition and population effect.