Registration Dossier
Registration Dossier
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EC number: 203-607-0 | CAS number: 108-69-0
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.01.1990 - 25.01.1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- "Gute Laborparxis" 1983
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Laboratory culture: yes
- Name and location of sewage treatment plant where inoculum was collected: 10L from OECD lab, 19.01.1990
- Pretreatment: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Nominal and measured concentrations:
- 100, 180, 320, 560, 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorphenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Remarks:
- Respiration inhibition
- Effect conc.:
- 914 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity of the test was checked by means of a graphical evaluation of the reference substance.
- Conclusions:
- The EC50(3h) on microorganisms for the test substance was 914 mg/L (nominal).
- Executive summary:
The test item was assessed in an test on toxicity to bacteria with 3,5-Xylidine according to ISO 8192-1986 (E), similar to OECD 209 and following GLP. Activated sludge was incubated for 3h to nominal test concentrations of 100, 180, 320, 560, 1000 mg/L- The respration rate was measured. The EC50(3h) on microorganisms for the test substance was 914 mg/L (nominal).
Reference
| Test item concentration (mg/L) | Respiration rate (mg/L*h) | Inhibition (%) |
100 | 24 | 7 |
180 | 24 | 7 |
320 | 24 | 7 |
560 | 18 | 31 |
1000 | 10 | 60 |
Description of key information
ISO 8192-1986 (E), similar to OECD 209, activated sludge, GLP, nominal test concentrations of 100, 180, 320, 560, 1000 mg/L- The EC50(3h) 914 mg/L (nominal).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 914 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
