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EC number: 608-307-7 | CAS number: 2901-13-5
Ecotoxicological information
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of experimental part: 06.08.1997 / End of experimental part: 06.08.1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: 1a: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- yes
Test material
- Reference substance name:
- α,α-Dimethyl-ethyl ester benzeneacetic acid
- EC Number:
- 608-307-7
- Cas Number:
- 2901-13-5
- Molecular formula:
- C12 H16 O2
- IUPAC Name:
- α,α-Dimethyl-ethyl ester benzeneacetic acid
- Details on test material:
- Name: Ethyl dimethylphenylacetate
Synonyms: EDMPA
Chemical name: Benzeneacetic acid, α,α-dimethyl-, ethyl ester
Code: HOE CG 0523 0Y ZD00 0001
Certificate of analysis: March 18, 1997
Purity: 99.8%
Appearance: clear, colorless liquid
Solubility: 0.3 g/L in water
Batch number: B 0006
Date of production: March 12, 1997
Date of expiry: March 18, 2000
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Origin: Sewage treatment plant at Frankfurt am Main-Sindlingen
Type of inoculum: Domestic activated sludge
Preparation of the suspension: sediment from activated sewage sludge was washed three times by centrifuging and resuspending in water with 0.9 %NaCl.
Amount used: 450 ml/l sludge suspension
Content of suspended solids: 27.78 g/l (centrifuged wet matter)
Content of suspended solids: 3.56 g/l (dry matter)
Content in the final test volume: 1.6 g/l (dry matter)
Pretreatment: corresponding to the cited test guideline.
State of acclimatization: not pre-adapted to the test substance or the reference substance.
Storage: The concentrated inoculum suspension was aerated over 2 days. Nutrition solution was added during this period.
Study design
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- The incubation temperature was controlled at 20.4°C to 21.2°C.
- pH:
- 6.9-7.5 after the incubation of the test batches.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glasbottles with a diameter of 60 mm and a grounded neck (NS 29), height 170 mm and a total volume of approx. 480 ml.
- Test volume: 200 ml
- Aeration/test vessel: 50L/hour only during the incubation period
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Justification for test concentrations: Due to the low solubility no stock solution was prepared. The test material was weighed out directly into the test vessels.
Highest concentration tested: 1000 mg/l, lowest concentration tested: 62.5 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol in the concentrations of 5, 20 and 30 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 186 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 27 to 351 mg/l
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 561 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 295 to 3342 mg/l
- Results with reference substance (positive control):
- Testing the respiration inhibition of activated sludge proceeded without disturbances. The EC50 of the reference substance 3,5-Dichlorophenol was between 5 mg/L and 30 mg/L and amounted to 10 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 of the substance to Activated sludge (domestic sewage treatment plant) is in the range >100 mg/l to 1000 mg/l.
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