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EC number: 608-307-7 | CAS number: 2901-13-5
Ecotoxicological information
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start of study: January 26 - End of study: January 28, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: 1a: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes
- Remarks:
- Principles of Good Laboratory Practice, annex of paragraph 19a, section 1 of the chemical law
Test material
- Reference substance name:
- α,α-Dimethyl-ethyl ester benzeneacetic acid
- EC Number:
- 608-307-7
- Cas Number:
- 2901-13-5
- Molecular formula:
- C12 H16 O2
- IUPAC Name:
- α,α-Dimethyl-ethyl ester benzeneacetic acid
- Details on test material:
- Name: EDMPA
Synonyms: Ethyldimethylphenylacetat, 2-Phenylisobutyrsäureethylester
Chemical name: 2-methyl-2-phenyl-propionic acid ethyl ester
Certificate of analysis: August 26, 1999
Purity: 99.8%
Appearance: clear, colorless liquid
Batch number: C 0086
Date of production: August 26, 1999
Date of expiry: August 26, 2000
Storage conditions: darkness at approximately 5°C in a refrigerator
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The samples were diluted with water.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: Stock culture of Aventis Pharma Deutschland GmbH, ProTox
- Feeding during test: daphnids were not fed during the entire study period.
- Food type: The supplied diet was based on the amount of organic carbon (C).
- Amount: The ration level is 0.2 mg carbon/day/animal.
- Frequency: three times a week with monocellular green algae (Scenedesmus subspicatus).
ACCLIMATION
- Feeding frequency: three times a week with monocellular green algae (Scenedesmus subspicatus).
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.3 mmol Ca²+ + Mg²+/L
- Test temperature:
- 21 ± 1 °C
- pH:
- Control group: 7.9-8.0
Controls: 7.8-7.9 - Dissolved oxygen:
- Control group: 8.8-9.3 mg/L
Controls: 8.9-9.1 mg/L - Nominal and measured concentrations:
- Nominal test concentrations: 0-10-22-50-100 mg/L
Mean measured concentrations: 0-12.6-20.6-47.4-85.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Material, size, headspace, fill volume: 250 ml beakers (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 ml test water.
- No. of organisms per vessel: 10 daphnids (=20/concentration) in each test chamber
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted fresh water was used for breeding and dilution. Demineralized water with a conductance of < 1 µS/cm was used for preparing the artificial fresh water (according to Elendt).
- Ca/mg ratio:6.57
- Conductivity: < 1 µS/cm
- Light intensity: The room was illuminated by fluorescent tubes for 16 hours each day.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 24h
- Test concentrations: 0/10/22/50/100 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 12.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 12.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 39.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Remarks on result:
- other: 32.1-47.0
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 36.9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
- Remarks on result:
- other: 30.4-43.9
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 33.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mobility
- Remarks on result:
- other: 27.8-39.6
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 31.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
- Remarks on result:
- other: 26.1-37.4
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 85.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 85.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48h) = 33.2 mg/l under the conditions of the test.
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