Methyl cinnamate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

N/A

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented, available information is sufficient for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl cinnamate

EC Number:

203-093-8

EC Name:

Methyl cinnamate

Cas Number:

103-26-4

Molecular formula:

C10H10O2

IUPAC Name:

methyl 3-phenylacrylate

Test material form:

solid: crystalline

Details on test material:

Chemical name Methyl cinnamate

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

New Zealand white rabbits of both sexes (weighing 2.5 to 3.0 kg.) were used.

Administration / exposure

Type of coverage:

open

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

The data obtained from acute oral LD50 studies in rats indicated relatively low toxicity of all test materials. Higher dosages were not used since LD50 values would have little meaning. Therefore, only one selected dose level of each compound was applied to a group of four rabbits of both sexes (weighing 2.5 to 3.0 kg). The dose site, approximately 240- cm2 (about 10% of body surface) was closely clipped. The skin of two of the animals was abraded and the skin of the two remaining animals was left intact. The skin was slightly moistened with physiological saline prior to application. Solid test materials were first ground to fine powders and then applied to the rabbit skin and covered with a nonabsorbent binder.

Duration of exposure:

24 hr

Doses:

5 g/kg bw

No. of animals per sex per dose:

2

Control animals:

yes, concurrent no treatment

Details on study design:

Animals were immobilized for 24 hours in stocks. Immediately following the removal of the binders, the local effects of the test compounds were scored according to the method of Draize. Toxic effects, dermal irritation, and mortality were recorded daily for 14 days. A necropsy was performed on each animal that died during the experiment and on those killed (by air embolism) at termination. Abnormal tissues and samples of adrenal, kidney, liver, lung, spleen, gonads, urinary bladder, bone, and skin were preserved in 10% formalin for possible histologic examination.

Statistics:

N/A

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No adverse effects were observed during the test.

Mortality:

There was no mortality observed with methyl cinnamate during the test.

Clinical signs:

other: All animals appeared to be normal throughout the entire 14-day observation period.

Gross pathology:

No gross pathologic findings occurred in the rabbit that were killed at termination.

Other findings:

N/A

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on the results given in this study, the dermal LD50 value is considered to be greater than 5000 mg/kg body weight.

Executive summary:

This study was conducted to investigate the acute dermal toxicity of test substance. Rabbits were applied at dosage level of 5 g/kg body weight. There was no mortality of methyl cinnamate applied animals during the test. All animals appeared to be normal throughout the entire 14-day observation period. No gross pathologic findings occurred in the rabbit that were killed at termination. There was no skin irritation to either intact or abraded skin following treatment with test substance.

Therefore, test substance failed to cause acute dermal toxicity to rabbits during the test period.