Poly(oxy-1,2-ethanediyl), .alpha.,.alpha.'-2-butyne-1,4-diylbis[.omega.-hydroxy-

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

The study is not assignable due to missing determination of the nominal concentration.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

(adopted on 1981, 12th May; Inhalation hazard test described in the Annex)

Deviations:

yes

Remarks:

(No details on animal husbandry, limited documentation, no determination of nominal and analytical concentration).

Principles of method if other than guideline:

BASF-internal standardized test method: the test was performed in principle as described in OECD test guideline 403. It demonstrates the toxicity of an atmosphere saturated with vapours of the volatile components of a test substance at 20°C and 50°C. Young adult laboratory rats, 3 per sex, were exposed sequentially to the vapours generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 8 h. The exposure was subsequently repeated in the same manner. No analytical determination of the atmosphere concentrations was performed. If possible, the nominal concentration was calculated as quotient of the amount of test substance weight loss during exposure, and the amount of air used during exposure. Group-wise documentation of clinical signs was performed over a 7 day study period. Body weight of groups was determined before the start of the study and at the end of the observation period.

GLP compliance:

no

Test type:

other: inhalation hazard test

Test material

Test material information

Constituent 1

Reference substance name:

Hydroxyethylated 2-butyne-1,4-diol

EC Number:

608-711-3

Cas Number:

32167-31-0

Molecular formula:

C4 H6 O2 (C2 H4 O) n, where 1 < n < 4.5

IUPAC Name:

Hydroxyethylated 2-butyne-1,4-diol

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): Golpanol SC 9189
- Physical state: liquid
- Analytical purity: no data

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Rats were exposed for 8 h to a vapour saturated atmosphere. Vapour was generated by bubbling 200 L/h air through the liquid substance column (volume ca. 50 mL) of about 5 cm above a fritted glass disc in a glass cylinder. The air pressure was 754 mm Hg. Temperature in the exposure chamber was 20 and 50 °C.

TEST ATMOSPHERE
- Brief description of analytical method used: no analytics performed
- Samples taken from breathing zone: no

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

Due to missing substance loss, test substance concentration could not be calculated.

No. of animals per sex per dose:

12

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: group wise documentation on working days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

other: Inhalation Hazard Test

Exp. duration:

8 h

Remarks on result:

other: No mortality.

Mortality:

No mortality observed.

Clinical signs:

other: No abnormality detected.

Gross pathology:

No abnormality detected.

Applicant's summary and conclusion

Executive summary:

In an acute inhalation hazard test comparable to OECD 403 (adopted on 1981, 12th May; inhalation hazard test), rats were whole body exposed to an atmosphere of saturated vapours of the test substance (analytical purity: not known) at 20 °C for 8 hours (6/dose/sex) or at 50°C for 8 hours (6/dose/sex) and observed for 7 days (BASF XXII/42, 1972). No nominal concentration could be calculated. No mortality and no clinical signs were observed. There were no data about body weight of single animals. In sacrificed animals acute bronchopneumonia was detected in 8/12 animals at 20°C and in 1/8 animals at 50°C.

These studies suggest that exposure ofto highly saturated vapours represents no hazard.