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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

EC50 (invertebrates)= > 100 mg/l, 48hour, freshwater, OECD 202, Migchielsen 2013

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information

The acute toxicity to Daphnia magna was carried out according to OECD 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. Twenty Daphnia magna per test group (4 replicates, 5 daphnia per replicate vessel) were exposed to untreated control medium and to a concentration of 100 mg/l in the limit test. Ten daphnia per test group (2 replicates, 5 per replicate) were exposed to 0.10, 1.0 and 10 mg/l in the combined range-finding test. Analysis showed that the measured concentration in samples taken from nominally 100 mg/l were in agreement with nominal at the start of the test and that they did not decrease by more than 20% during the test period. Results were consequently based on the nominal concentration. No or no significant effects on the mobility of Daphnia magna were observed up to and including 10 mg/l, while an average of 35% immobile daphnia were observed at 100 mg/l. There was no significant variation in the results of the combined limit/range-finding test and there were no significant responses at lower concentrations, furthermore test item concentrations were satisfactorily maintained during the course of the study. The 48h-EC50 was >100 mg/l based on analytically confirmed nominal concentration.