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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to EU/OECD-guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2014

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
sodium 4-(methoxycarbonyl)-5-oxo-2,5-dihydrofuran-3-olate
EC Number:
627-063-2
Cas Number:
1134960-41-0
Molecular formula:
C6H5O5Na
IUPAC Name:
sodium 4-(methoxycarbonyl)-5-oxo-2,5-dihydrofuran-3-olate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
in deionised water

Test organisms

Test organisms (species):
other: mixed population of aquatic microorganisms (activated sludge)

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 ± 2°C
pH:
8
Nominal and measured concentrations:
1000 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
>= 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item showed 0.00 % respiration inhibition of activated sludge at the highest test item concentration of 1000 mg/L.
The EC50 is higher than 1000 mg/L.
The NOEC is equal or higher than 1000 mg/L.