Tetrachlorophthalic anhydride

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1958

Report date:

1958

Materials and methods

Principles of method if other than guideline:

BASF-Test

Before OECD Guideline 405 was established, eye irritation was tested using an internal method (BASF test). White Vienna rabbits were used. 0.1 g of the test substance was applied to the conjunctival sac of the right eye of the animal. The adjacent eye was treated with one drop of physiological solution of sodium chloride and served as a control. The animals were observed several times on the treatment day and 8 days afterwards, and findings were recorded on working days.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachlorophthalic anhydride

EC Number:

204-171-4

EC Name:

Tetrachlorophthalic anhydride

Cas Number:

117-08-8

Molecular formula:

C8Cl4O3

IUPAC Name:

tetrachloro-1,3-dihydro-2-benzofuran-1,3-dione

Details on test material:

- Name of test material (as cited in study report): Tetrachlorophthalic anhydride
- Analytical purity: no data

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.83-3.32 kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: The adjacent eye was treated with one drop of physiological solution of sodium chloride and served as a control.

Amount / concentration applied:

0.1 g

Duration of treatment / exposure:

8 days

Observation period (in vivo):

8 days

Number of animals or in vitro replicates:

2

Details on study design:

SCORING SYSTEM: The original BASF grading was converted into the numerical grading according to the OECD Draize system.

Results and discussion

Any other information on results incl. tables

One hour after application of the test substance slight erythema, secretion, residues of test substance and a film on the cornea was reported for both animals. 24 hours after application of the test substance, secretion, corneal opacity, slight erythema, and slight edema was reported for both animals. On day 2, only slight erythema was left in one animal, which reversed fully until day 8.

Animal	Readings	Cornea	Conjunctivae (Redness)
01	24 h	2	1
	48 h	0	1
02	24 h	3-4	1
	48 h	0	1