Tetrachlorophthalic anhydride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, without analytical monitoring.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachlorophthalic anhydride

EC Number:

204-171-4

EC Name:

Tetrachlorophthalic anhydride

Cas Number:

117-08-8

Molecular formula:

C8Cl4O3

IUPAC Name:

tetrachloro-1,3-dihydro-2-benzofuran-1,3-dione

Details on test material:

- Name of test material (as cited in study report): Tetrathal
- Analytical purity: not stated

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Concentration of the stock solution was 250 mg/l. Delution series with dilution water gave the nominal test concentrations:

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.54 mmol/l

Test temperature:

20+/- 1°C

pH:

7.25- 8.1
(250 mg/l-not neutralized): 4.3-5.1)

Dissolved oxygen:

> 2 mg/l

Nominal and measured concentrations:

Nominal test concentrations mg/l:
250, 125, 62.4, 31.2, 15.6, 7.8, 3.9, 1.95, 0.975 (Additionally the highest concentration (250 mg/l) has been tested after neutralization.)

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 ml glass tubes with flat bottom
- Fill volume: 10 ml test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Ca/mg ratio: ca. 4:1


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (5 microeinstein/m*m*s(400-750nm))


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Ph values and dissolved oxygen: after 0 and 48 hours
Mobility of daphnids: after 0, 3 ,6, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

154 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The product will cause changes in the pH Value of the test system (250 mg/l = pH 4.37). After neutralisation a reduction in harmful effect can be observed. Mortality after 48h (250 mg/l) - neutralized = 10 % Mortality after 48h (250 mg/l) - not neutralized = 100 %

Any other information on results incl. tables

Concentration response relationship (not neutralized):
                             mobile daphnids
Concentrations     / 0h / 3h  /  6h  /  24h  /  48h     
control (0mg/l)      20   20     20     20      20
  0.975              20   20     20     20      20 
  1.95               20   20     20     20      20
  3.9                20   20     20     20      20
  7.8                20   20     20     20      20
 15.6                20   20     20     20      20
 31.2                20   20     20     20      19
 62.4                20   20     20     20      19
125                  20   20     20     20      18
250                  20    0      0      0       0