Sodium dodecylbenzenesulfonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Toxicologic studies with branched and linear alkyl benzene sulfonates in rats

Author:

Oser BL and Morgareidge K

Year:

1965

Bibliographic source:

Toxicology and applied phamacology 7, 819-825

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Hagan, EC (1959). Acute toxicity. In: Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics. pp. 17-25. Assoc. of Food and Drug Officials of the US, Bureau of Food and Drugs, Texas State Dept. of Health, Austin, Texas.

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Benzenesulfonic acid, alkyl derivs.

Cas Number:

42615-29-2

IUPAC Name:

Benzenesulfonic acid, alkyl derivs.

Test material form:

solid: compact

Details on test material:

LAS had a nominal chain length of 12 carbon atoms (range C9-C15) and an average molecular weight of 346.
(Benzenesulphonic acid, linear alkyl) (C9-C15), purity 39.5% (sodium sulphate 8.8%, water 50.9 %, free alkali (NaOH) 0.05%, unidentified 0.64%)

Test animals

Species:

rat

Strain:

other: FDRL strain(Wistar-derived)

Sex:

male/female

Details on test animals or test system and environmental conditions:

young adult rats : FDRL strain (Wistar-derived)They were fasted overnight before this test.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

10% and 40 % dispersions of the samles were prepared in distilled water and administered intragastrically.

Doses:

10% and 40% dispersion of LAS sample at dosages of 0.6 and 1.58 g/kg.

No. of animals per sex per dose:

3

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations for behaviour, appearance and survival were made daily; weighings were performed on days 0, 7, and 14
- Necropsy of survivors performed: yes

Statistics:

LD50 calculated by the method of Miller, LC, and Tainter, ML. (1994). Estimation of the ED50 and its error by means of logarithmic-probit graph paper. Proc. Soc. Exptl. Biol. Med. 57, 261-264.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

650 mg/kg bw

Based on:

act. ingr.

95% CL:

> 587 - < 713

Mortality:

Motaliity was not affected.

Clinical signs:

other: A high incidence of diarrhea was noted with the use of the higher concentration. Those rats which succumbed appeared to be weak and showed reduced voluntary activity prior to death.

Gross pathology:

No significant gross abnormalities were seen at autopsy.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Acute oral LD50of LAS is 650 +/- 63 mg/kg (with a slope factor of 0.173) when the samples are administered in water dispersion to rats.
The acute oral LD50 in male/female rats is 650mg/kg bw. No significant gross abnormalities were seen at autopsy.

Executive summary:

An acute oral toxicity study was performed for linear alkylbenzene sulphonate (LAS: nominal chain lengh of 12 carbon atoms and an average molecular weight of 346) using rats (FDRL strain, 3/sex/dose) for 14 days. 10% and 40% dispersions of the LAS were prepared in distilled water and administered intragastrically. The animals were observed several times daily for behavior, appearance, and survival for a 14-day period. They were weighed initially and at 7 and 14 days. All animals that died on test as well as those sacrificed at the conclusion of the observation period were necropsied. The acute oral LD₅₀ in rats is 650mg/kg bw.No significant gross abnormalities were seen at autopsy.