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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999-04-06 to 1999-04-09
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted 4 April 1984
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Official Journal No. L 383 A, 29 December 1992
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dihydroxyacetone
EC Number:
202-494-5
EC Name:
1,3-dihydroxyacetone
Cas Number:
96-26-4
Molecular formula:
C3H6O3
IUPAC Name:
1,3-dihydroxyacetone
Details on test material:
Name: Dihydroxyacetone

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item (reconstituted water and test item) was freshly prepared. The test item was weighed into a calibrated flask and filled with reconstituted water. After stirring of 10 minutes, the test item was completely dissolved and the flask filled to the mark with reconstituted water.
- Controls: reconstituted water for Daphnia (ELENDT M4)
- Concentration of the test item in the test medium: 100 mg/L

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Daphnia magna (Straus)
- Age at study initiation: not older than 24 hours
- Method of breeding: The parent strain (stock) was bred and maintained in vessels containing many daphnids in different ages.
For a new study, young daphnids were separated in 100 mL of reconstituted water. Once a week, the medium was renewed and the daphnids were fed with fresch water algae. Newborn animals were separated and used for the acute immobilization study.
- Source: Test Facility
- Feeding during test : No

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg/L expressed as CaCO3
Test temperature:
Start of test: 22 °C
End of test: 21 °C
pH:
Start of test: pH 8.0
End of test: pH 7.9 - 8.0
Dissolved oxygen:
Start of test: 96.9 - 99.6 %
End of test: 94.4 - 96.2 %
Nominal and measured concentrations:
Nominal concentration: 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 25 mL glass vessel
- Type (delete if not applicable): open
- Volume of solution: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light - 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The mobility of the daphnids was determined after 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilization observed in the pretest with 100 mg/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: No
- Immobilisation of control: No
- Abnormal responses: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No

Any other information on results incl. tables

Clinical findings

The nominal concentration and immobilization data of the study are shown in thefollowing table:

nominal

concentration

g/L

dead or immobilized/ exposed Daphnia magna

24 hours                            48 hours

Control

0 / 20

0 / 20

0.1

0/20

0/20

Daphnia exposed to the nominal concentration of 0.1 g/L (limit test) were not immobilized.

No remarkable observations were made concerning the appearance of the test material. All test media were clear preparations and stayed unchanged throughout the study.

EC50-value

Under the given experimental conditions, the test material showed a 48 h EC50 value of > 0,1 g/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
OECD 202 validity criteria fulfilled
Conclusions:
Under the given experimental conditions, the 48 h EC50 value of the test item exceeded the limit dose of 100 mg/L
Executive summary:

The objective of this study was to determine the acute toxicity of the test material to Daphnia magna. For this purpose, young Daphnia magna were exposed to an aqueous test material concentration under defined conditions. The study comprised of four vessels per concentration containing five Daphnia magna , i.e. 20 Daphnia per concentration (test medium group). The Daphnia were observed for immobilization up to 48 hours. The study was started with the exposure of Daphnia to the test material concentration of nominal 0.1 g/L in an open system. An analytical determination of the concentration was not performed.

Daphnia exposed to the test item at a nominal concentration of 100 mg/L were not affected.

According to the tested nominal concentrations the following values were obtained

24h EC50

> 100 mg/L

48h EC50

> 100 mg/L

Under the given experimental conditions, the test material showed a 48 h EC50 value exceeding the limit dose of 100 mg/L.