Registration Dossier

Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
other: EU Risk Assessment
Adequacy of study:
other information
Reliability:
other: EU Risk Assessment
Rationale for reliability incl. deficiencies:
other: No reliability is given as this is a summary entry for the EU RAR.

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Unnamed
Year:
2003
Reference Type:
publication
Title:
Biodegradation of 1,2,3- and 1,2,4-trichlorobenzene in soil and in liquid enrichment culture.
Author:
Marinucci AC and Bartha R
Year:
1979
Bibliographic source:
Appl. Environ. Microbiol., 38(5), 811-817.
Reference Type:
publication
Title:
Toxicity of chlorobenzenes to a lux-marked terrestrial bacterium, Pseudominas fluorescens.
Author:
Boyd EM et al.
Year:
1998
Bibliographic source:
Envir. Toxicol. Chem., 17, 2134-2140.

Materials and methods

Principles of method if other than guideline:
EU Risk Assessment
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Results and discussion

Any other information on results incl. tables

EU Risk Assessment (2003):

1,2,4-TCB was toxic to soil microbiota as CO2 development was reduced at increasing concentrations. At 50µg/g sandy soil, the total CO2 evolution was depressed to approximately 50% of the normal and the turnover optimum measured as percent 1,2,4-TCB degradation was

between 10 and 25µg/g during a 24-hour study period (Marinucci and Bartha, 1979).

The toxicity of 1,2,4-TCB on terrestrial microorganisms was studied on a terrestrial bacterium by insertion of lux genes into the genome of Pseudominas fluorescens. The bioluminiscence was determined after 20 minutes of exposure to mono-, di-, tri-, tetra-, penta- and hexachlorobenzene. The toxicity increased with the chlorination. For 1,2,4-TCB, the EC50 was 18.3 mg/l (Boyd et al., 1998). The test was performed with a soil bacteria, but in a short-term test in an aqueous solution.

Applicant's summary and conclusion