Methyl isothiocyanate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP Guideline study (OECD 404)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA - Pesticide assessment guidelines

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl isothiocyanate

EC Number:

209-132-5

EC Name:

Methyl isothiocyanate

Cas Number:

556-61-6

Molecular formula:

C2H3NS

IUPAC Name:

isothiocyanatomethane

Details on test material:

Test article name : METHYLSENFOEL (MITC)
Source: DEGUSSA AG
Purity: technical

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Kleintierfarm madoerin AG (CH 4414 Fuellinsdorf, Switzerland)
- Age at study initiation: 15-16 week-old
- Weight at study initiation: 2.4/2.6 kg
- Housing: individually in stainless steel cages equipped with an automatic cleaning and drinking system.
- Diet (e.g. ad libitum): pelleted standard kliba 341, ad libitum
- Water (e.g. ad libitum):Tap water, ad libitum
- Acclimation period: 4 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-2
- Humidity (%): 55+/-10%
- Air changes (per hr): 10-15
- Photoperiod (hrs dark / hrs light):12/12 (8h music/light period)

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml
- Concentration (if solution): no data

no VEHICLE

Duration of treatment / exposure:

4 hours

Observation period:

4 hours

Number of animals:

3 = 1 male + 2 females

Details on study design:

TEST SITE
- Area of exposure: the dorsal
- % coverage: patch = 3x3cm
- Type of wrap if used: Skin was recovered with a patch of surgical gauze. The gauze was covered with aluminium foil and then covered with an occlusive dressing. The dressing was wrapped around the abdomen and anchored with an elastic bandage.

REMOVAL OF TEST SUBSTANCE :
Just after the animals death (approximately 1 hour after treatment), the dressing was removed.

SCORING SYSTEM: idem in guideline of OECD 404

Results and discussion

In vivo

Results

Remarks on result:

other: All animals died within the 4-hour treatment phase, therefore no irritation index could be determined

Irritant / corrosive response data:

All animals died within the 4-hour treatment phase, therefore no irritation index could be determined. The index given below was determined just after removing the occlusive bandage, when the animals were already dead. After 1 hour: for each animal, erythema score = 4 and edema score = 2. After 4 hours: all animals died.

Other effects:

Toxic symptoms/mortality: Between one and four hours after application start all animals showed sedation, crying, spasms, dyspnea and curved body position.

Applicant's summary and conclusion

Interpretation of results:

other: unconclusive

Conclusions:

All animals died within the 4 -hour treatment phase, therefore no irritation index could be determined.

Executive summary:

The purpose of this primary study skin irritation study was to assess possible irritation potential when single doses of MITC are placed on the skin on rabbits. All animals died within the 4 -hour treatment phase, therefore no irritation index could be determined.