Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
other: No endpoint assignable
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: No effects at water saturation of test item

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The inbibitory effect of the test item Fluowet I 800 on the respiration rate of aerobic wastewater miccroorganisms of activated sludge was investigated in a 3 -hour respiration inhibition test according to the OECD Guideline 2009. In accordance with the EU Commission Directive 92/69/EEc (C.1, C.2 and C.3), a limit test with a nominal test concentration of 1000 mg/L was performed.

In addition, two controls and three different concentrations of the reference item 3,5 -dichlorphenol (5, 16 and 50 mg/L) were tested in parallel. the results of these treatments confirmed suitability of the activated slufge and the method used.

The test item had no significant inhibitory effect (< 15%) on the respiration rate of activated slufge after the incubation period of three hours at the test item concentration of 1000 mg/l.

Thus, the 3 -hour NOEC (EC15) of the test iten to activated sludge microorganisms was at least 1000 mg/L. this value might even be higher, but concentrations above 1000 mg(L were not tested. The 3 -hour EC20, EC50 and EC80 could not be calculated but were clearly higher than 1000 mg/L