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Environmental fate & pathways

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A study was conducted to assess the ready biodegradability of the test substance according to EU Method C.4-B, in compliance with GLP. Activated sludge was inoculated with 19.1 mg/L (expressed in terms of dissolved organic carbon (DOC)) of the test substance (in duplicate) and incubated under aerobic conditions at a constant temperature (22±2°C) for 28 d. Degradation was assessed by the determination of DOC on Days 0, 7, 14, 21, 27 and 28. A control solution, a reference test (containing the reference substance benzoic acid, sodium salt (19.8 mg/L DOC) together with inoculum) and a toxicity test (containing the test substance, reference substance and the inoculum) were used for validation purposes. The test substance degraded 0% by Day 28. No toxicity was observed in the toxicity control. The reference substance showed 97% degradation at Day 14. Under the study conditions, the test substance was considered to be ‘not readily biodegradable’ (Müller, 2000).