Dilithium disodium (5,5'-diamino-(μ-4,4'-dihydroxy-1:2-κ-2,O4,O4',-3,3'-[3,3'-dihydroxy-1:2-κ-2-O3,O3'-biphenyl-4,4'-ylenebisazo-1:2-(N3,N4-η:N3',N4'-η)]-dinaphthalene-2,7-disulfonato(8)))dicuprate(2-)

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-01-14 to 1992-06-02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline-conform study under GLP without deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 202 pt II, adopted April 1984.

Deviations:

no

Principles of method if other than guideline:

OECD Guideline for the Testing of Chemicals 202, Part II: "Reproduction Test at least 14 Days", Paris/France, adopted April 1984.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dilithium disodium (5,5'-diamino-(μ-4,4'-dihydroxy-1:2-κ-2,O4,O4',-3,3'-[3,3'-dihydroxy-1:2-κ-2-O3,O3'-biphenyl-4,4'-ylenebisazo-1:2-(N3,N4-η:N3',N4'-η)]-dinaphthalene-2,7-disulfonato(8)))dicuprate(2-)

EC Number:

407-230-4

EC Name:

Dilithium disodium (5,5'-diamino-(μ-4,4'-dihydroxy-1:2-κ-2,O4,O4',-3,3'-[3,3'-dihydroxy-1:2-κ-2-O3,O3'-biphenyl-4,4'-ylenebisazo-1:2-(N3,N4-η:N3',N4'-η)]-dinaphthalene-2,7-disulfonato(8)))dicuprate(2-)

Cas Number:

126637-70-5

Molecular formula:

Hill formula: C32 H16 Cu2 Li2 N6 Na2 O16 S4 CAS formula: C32 H16 Cu2 N6 O16 S4. 2Li 2Na

IUPAC Name:

dicopper(2+) dilithium(1+) disodium 4,4'-bis[2-(8-amino-1-oxido-3,6-disulfonatonaphthalen-2-yl)diazen-1-yl]-[1,1'-biphenyl]-3,3'-bis(olate)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

not applicable

Test conditions

Hardness:

14.2 - 15.0 dH°

Test temperature:

19.3 - 20.4 °C

pH:

The initial pH of the systems was, on average, 7.9 for untreated, 7.8 and 7.9 for the treated samples. At the end of the exposure period, the pH for the respective concentrations were 8.4, 8.5 and 8.4.

Dissolved oxygen:

The initial oxygen concentration was, on average, 8.7 mg oxygen/l for untreated and for the treated samples. At the end of the exposure periods, the oxygen concentration of the respective samples were 8.3, 8.7 and 8.5 mg oxygen/l.

Salinity:

Conductivity 584 µs/cm

Nominal and measured concentrations:

nominal 0.625 mg/l: below lowest detected concentration of 1 mg/l
nominal 2.5 mg/l: mean measured 2.355 mg/l (94.2 % recovery rate)
nominal 5.0 mg/l: mean measured 4.559 mg/l (91.2 % recovery rate)
nominal 10.0 mg/l: mean measured 9.098 mg/l (90.1 % recovery rate)
recovery rates 79.6 % - 108.6 % of nominal concentration

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 3

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 4

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

immobilisation

Results with reference substance (positive control):

The quality of the animals used is checked by determining the EC 50 value for potassium dichromate twice per year, however no data reported.

Reported statistics and error estimates:

The results were statistically evaluated using the Steel-test.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC- and LOEC-values for daphnia reproduction rate (21 days) were found to be ≥ 10 mg/l and >10 mg/l, respectively.