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EC number: 942-100-5 | CAS number: -
- Life Cycle description
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- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 31, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "BESTIMMUNG DER WIRKUNG VON WASSERINHALTSSTOFFEN AUF FISCHE - FISCHTEST (L15)"
- Deviations:
- no
- GLP compliance:
- no
- Details on sampling:
- - Concentrations: 100, 215, 464, 1000, 2150 and 4640 mg/l
- Sampling method: The product was added to the test water without any pretreatment - Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- TEST ORGANISM
- Common name: Golden Orfe
- Source: Fischucht Eggers D-W2354 Hohenwestedt, FRG
- Length at study initiation (length definition, mean, range and SD): 7.3 cm (range: 6.8 - 8.1)
- Weight at study initiation (mean and range, SD): 3.7 g (range: 2.3 - 5.2)
- Method of breeding: ad libitum
- Food type: Growing feed F/B 50
- Corpulence factor: 0.9
ACCLIMATION
- Acclimation period: about 1 month
- Acclimation conditions (same as test or not): same as test
- Mortality during the last 2 weeks of housing: 0.1%
- Mortality during the adaptation period: 0%
- Medical treatment: a few days after arrival prophylactical treatment (twice with 0.05 mg/l malachite green chloride and once with 10 mg/l tetracycline hydrochloride)
- Adaptation to test water and test conditions: 3 days
- Withdrawal of food: 1 day before and during exposure - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/l
- Test temperature:
- 20-21 °C
- pH:
- about 8.0
- Dissolved oxygen:
- > 60% of maximum saturation
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All-glass aquarium (30 cm x 22 cm x 24 cm)
- Material, size, headspace, fill volume: flow-throught tank in tap water cleaned by active carbon
- Volume: 10 l
- Aeration: continuous aeration with oil-free air
- Photoperiod: 16h light and 8h darkness
- No. of organisms per vessel: 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the product was added to the test water without any pretreatment; subsequently the fish were placed into the aquaria.
- Ca/mg ratio: 4:1
- Na/k ratio: 10:1
- Acid Capacity: 0.8 mmol/l
TEST CONCENTRATIONS
based on the results of a range finding study (LC 50 after 96 h: between 100 and 1000 mg/l) and a main study not evaluated the concentrations, spaced by a factor of about 2.2, were fixed as follows: 100, 215, 464 and 1000 mg/l.
Since no LC 100 had been achieved the additional concentrations 2150 and 4640 mg/l were added 1 week later. - Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 792 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 215 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- ca. 215 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- ca. 2 150 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Observed symptoms: Apathy, Narcotic-like state, Tumbling
- Results with reference substance (positive control):
- LC50 (48h) = ca. 32 mg/l
- Reported statistics and error estimates:
- Determination or calculation of the median lethal concentration (LC50) and, if possible the LC5 and the LC95 using the probit analysis[1] after hours (nominal conc): 1, 4, 24, 48, 72 and 96
[1]: "Probit analysis", Finney, d.j., Cambr. Univ. Press, 3rd ed., 1971 - Sublethal observations / clinical signs:
Summary results:
LC50 (mg/l; nominal concentrations) after: 1h > 4600 mg/l 4h > 2200; < 4600 mg/l 24h > 1000; < 2200 mg/l 48h ca. 1000 mg/l 72h ca. 1000 mg/l 96h = 792 mg/l slope factor = 1.49 - Validity criteria fulfilled:
- not specified
- Conclusions:
- LC50 (96h) = 792 mg/l
- Executive summary:
The substance to be registered has been tested for acute fish toxicity according to DIN 38 412 and has shown a NOEC = 215 mg/l, LC0 = 215 mg/l, LC50 = 792 mg/l and LC100 = 2150 mg/l.
Reference
Description of key information
Freshwater: LC50(96h) = 792 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 792 mg/L
Additional information
According to the CLP Regulation (EC) n. 1272/2008 ‘acute (short-term) hazard’ means for classification purposes the hazard of a substance or mixture caused by its acute toxicity to an organism during short-term aquatic exposure to that substance or mixture.
The test substance has been tested for acute fish toxicity. The selected key study shows a LC50 = 792 mg/l, this result is confirmed by the other available tests. The Huntsman test (1973) indicates a lower value (302 ppm after 96 h) but no complete information about the sample composition is available.
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