2-Butene, 1,1,4,4-tetramethoxy-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14.11.- 12.12.1990

Reliability:

1 (reliable without restriction)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1991

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

Principles of method if other than guideline:

not relevant

GLP compliance:

yes

Remarks:

statement of compliance

Test material

Test material information

Constituent 1

Reference substance name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

EC Number:

900-110-7

Cas Number:

5370-08-1

Molecular formula:

C8H16O4

IUPAC Name:

(2E)-1,1,4,4-tetramethoxybut-2-ene; (2Z)-1,1,4,4-tetramethoxybut-2-ene

Details on test material:

- Name of test material (as cited in study report): C4-Diacetale
- Physical state: colourless liquid
- Analytical purity: 97.1%

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

No further details

Any other information on results incl. tables

No remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

No physico-chemical degradation was observed when the test substance was incubated without inoculum.
Thus, C4-Diacetal may be characterized as: not easily biodegradable.

Executive summary:

The ready biodegradability of C4-Diacetal has been examined using the modified MITI test I. A mixture of activated sludge from a domestic sewage works and from a pilot chemical waste water treatment plant was exposed under controlled conditions to 100 mg/l of the test substance. After an incubation period of 28 days, the BOD/ThOD ratio was calculated to determine the biological degradability. C4-Diacetal shows a ready biodegradability of <1%. Control experiments revealed that the reference substance sodium benzoate was ultimately biodegraded and that the test substance had no toxic effect on the microogranisms in the activated sludge at concentrations >= 100 mg/l. No physico-chemical degradation was observed when the test substance was incubated without inoculum. Thus, C4-Diacetal may be characterized as: not easily biodegradable.