1-methylpiperidine

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Mechanism of DNA strand breakage by piperidine at sites of N7-alkylguanines.

Author:

Matthes B.W. et al.

Year:

1986

Bibliographic source:

Biochemica et Biophysica Acta 868, 71-76

Materials and methods

Type of study / information:

The effect of the test substance on the strand breaking ability on N7-alkylguanine was reported.

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

DNA was digested with a restriction enzyme, 32P-labeled and digested with a second restriction enzyme. These fragment were separated using an agarose gel and fractions containing radioactivity were pooled. After further processing breaks at sites of N7-guanine alkylation were created by resuspending the DNA with piperidine and other amines. Using a polyacrylamide gel electrophoresis, the average number of breaks per DNA molecule was determined.

Test material

Test material information

Constituent 1

Reference substance name:

N-Methyl Piperidine

IUPAC Name:

N-Methyl Piperidine

Results and discussion

Any other information on results incl. tables

The mechanisms proposed by Maxam and Gilbert (Methods Enzymol. 65, 499 - 560, 1980) that aqueous piperidine creates strand breaks at sites of N7-guanine alkylations was confirmed. It was shown that alkaline conditions catalyze rupture of the C8-N9 bond, forming a formamido-pyrimidine structure which is displaced from the ribose moiety by piperidine. High pH itself will not create strand breaks at sites of N7-alkylguanines. Additionally, they demonstrated that a primary or secondary amine as piperidine is required; a tertiary amine like N-Methyl Piperidine did not induce the formation of strand breaks in alkylated DNA.