Phthalic acid

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD guideline or GLP defined

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1942

Materials and methods

Principles of method if other than guideline:

Intraperitoneal injections of phthalic acid were given to 3-months-old, male, white mice. Six mice were kept in one cage; 4 such groups were simultaneously injected with a given dosage using a tuberculin syringe. The mice ranged from 18-22 g in weight; average weight: 20g. The mice were observed periodically and the total number dead at the end of 24 hours was taken as the kill for a given dosage. All of the mice surviving 24 hours recovered. The phthalic acid was given as a saturated solution which was shown to contain 0.01 g of phthalic acid per ml. Higher doses than 12 mg per mouse were not feasible since this dosage requred the intraperitoneal injection of 1.2 ml which is a large volume compared to the size of the peritoneal cavity.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Phthalic acid

EC Number:

201-873-2

EC Name:

Phthalic acid

Cas Number:

88-99-3

Molecular formula:

C8H6O4

IUPAC Name:

benzene-1,2-dicarboxylic acid

Details on test material:

no data

Test animals

Species:

mouse

Strain:

other: white mice

Sex:

male

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Doses:

6, 8, 10, 12 mg/kg

No. of animals per sex per dose:

24-41 mice

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 0.55 other: g/kg bw

Any other information on results incl. tables

Data on Dosage and Percentage Mortality:

	No. mice	Dosage	No. dead	Mortality %
	24	6 mg	1	4
	24	8 mg	11	44
	41	10 mg	19	46
	24	12 mg	12	50

Applicant's summary and conclusion

Executive summary:

In an acute toxicity study intraperitonel injections of Phthalic Acid (PA) were given to 3 -months-old, male mice. The PA was given as a saturated solution which was shown to contain 0.01 g of PA per ml. There was some cyanosis noted; the mice hopped about spasmodically, standing on their hind legs. In certain mice, autopsy showed an apparent subcutaneous hemorrhage in the nasal region; the livers exhibited white spots of unknown etiology.

The dose required to kill the average mouse (LD 50) was calculated to be 0.55 g per kg body weight.