Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 442-680-5 | CAS number: 443688-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 14th to September 11th, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Principles of method if other than guideline:
- the test method was modified to quantify the algicidal effect of colored test substances, but also the growth inhibition effect caused by reduced light intensities in the colored test media
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Red LF 6339
- IUPAC Name:
- Red LF 6339
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- samples were taken just before test start (duplicate samples from the single test medium and duplicate samples from the control without algae)
after 72 hours - stability samples (duplicate samples from the single test medium and duplicate samples from the control without algae)
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus Subspicatus CHODAT
- Strain: 86.81 SAG
- Source (laboratory, culture collection): supplied by the Collection of Algal Cultures (SAG), Institute for Plant Physiology, University of Gottingen, Germany
- Method of cultivation: grown in the laboratory under standardised conditions according to the test guidelines. Cultivated in synthetic test water prepared according to the test guidelines.
ACCLIMATION
exponentially growing pre-culture was used, which was set up 3 days prior to the test under conditions as in the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 0.24 mmol/l as CaCO3
- Test temperature:
- 23-24 °C
- pH:
- 7.9-8.1 (start)
8.0-8.3 (end) - Nominal and measured concentrations:
- 1, 3.2, 10, 32 and 100 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (50ml), each filled with 15 ml algal suspension continously stirred by magnetic stirrers. Each flask was placed in a black cylinder, coated inside witth aluminium foil
- Type (delete if not applicable): on the top of each cylinder glass dishes covered with watch glass dishes were placed to reduce the loss of water
- Initial cells density: 10000 algal cells/ml test medium
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water as recommended in the test guideline
- Intervals of water quality measurement: pH was measured in each test concentration and the control at the start and at the end of the test. The water temperature was measured daily in an Erlenmeyer flask filled withwater and incubated under the same conditions as the test flask. The appearance of the media was recorded daily.
OTHER TEST CONDITIONS
- Photoperiod: continously illuminated
- Light intensity and quality: 8217 lux (mean value). The light intensity was measured just before the start of the test below the coating cylinders.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: small volumes of the test media and the control were taken out of all test flasks after 24, 48 and 72 hours of exposure. The algal cell densities were determined by counting with an electronic partcile counter, with at least 2 measurements per sample. in addition, after 72 hours exposure, a sample was taken from the control and from a test concentration with reduced algal growth in experimental part A (nominal 32 mg/l). The shape of the algal cells were microscopically examined in these samples. For the quantification of the algicidal effect versus the growth inhibition due to reduced light intensitied, the percentage inhibition of algal biomass and growth rate (compared to the control in part A) was calculated for each concentration. The growth rate was compared between parts A and B.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
Experimental Part A
The algae grew in the test media with dissolved test item in the Erlenmeyer flasks (five test concentrations and a control). All glass dishes above the cylinders contained purified water. Thus the inhibition of algal growth in this part was caused by the toxic effect of the test item an in addition by the reduced light intensities in the coloured test media in the flasks
Experimental Part B
The glass dishes above the cylinders contained the colored test media at the same test concnetrations as in part A, but without algae (3 replicates per test concentration). In the flasks below, the algae grew in test water without test item (as in the control), however, under changed light conditions, due to the filter effect of the colored test media in the glass dishes. Thus, the growth in part B, was caused by light absorption only.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 114.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: this EC-value and its 95 % confidence limits should be taken with caution, since the inhibition of r was up to the highest test concentration just 50 %.
- Remarks:
- experimental part A
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: experimental part A
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: experimental part A
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: this EC-value should be taken with caution, since the inhibition of r was up to the highest test concentration only 21.4 %
- Remarks:
- experimental part B
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 29 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: experimental part B
- Details on results:
- The test item was stable during the 72-hrs test period under the test conditions.
Experimental Part A: test item had a statistically significant inhibitory effect on the growth (biomass and growth rate) after the exposure period of 72 hrs at the concentration of 3.2 mg/l. This test concentration was determined as the 72-hr LOEC. The 72-hr NOEC was determined as 1.0 mg/l. At the microscopical examination, the shape of algae cells after the 72-hr incubation, showed no difference between the algae growing in the test concentration of 32 mg/l and the algal cells in the control.
The observed growth inhibition of the test item on algae was caused in part due to the indirect effect, the light absrption in the colored test solution. However, the differences between experimental parts A and B were too high to state that the algal growth is inhibited solely as a result of a reduction in light intensity. Thereforem the results of experimental part A should be taken into consideration for the detemination of the toxic effect of the test item on the growth of the algae.
All test media were slightly colored by the test item
Exponential growth in the control (for algal test): yes
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50 (72h, growth rate) = 114.6 mg/l (nominal)
EC10 (72h, growth rate) = 3.4 mg/l (nominal)
NOEC (72h, growth rate) = 1.0 mg/l (nominal)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.