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EC number: 619-635-5 | CAS number: 12567-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: other:
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21st November-15th December 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OECD guideline with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetrakis(isobutyrato)zirconium
- EC Number:
- 619-635-5
- Cas Number:
- 12567-73-6
- Molecular formula:
- Hill formula: C16H28O8Zr CAS formula: C16H28O8Zr
- IUPAC Name:
- Tetrakis(isobutyrato)zirconium
- Reference substance name:
- Zirconium, tetrakis(isobutyrato)- (8CI)
- IUPAC Name:
- Zirconium, tetrakis(isobutyrato)- (8CI)
- Details on test material:
- - Name of test material (as cited in study report): Zirkonium-tetrakis(isobutyrato)
- Physical state: solid
- Analytical purity: 97.4 % (complexometric titration)
- Impurities (identity and concentrations): By-products:
toluene: ca. 2.1 %.
iso-butyric acid: ca. 0.5 %.
- Lot/batch No.: 02/05 (HK1005)
- Expiration date of the lot/batch: September 2006
- Storage condition of test material: Ambient temperature. Tightly closed. Storage under a nitrogen atmosphere, as the substance reacts with water.
Constituent 1
Constituent 2
Method
- Target gene:
- His
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Metabolic activation:
- with and without
- Metabolic activation system:
- microsomal fraction of homogenised livers of female Sprague Dawley rats treated once with 500 mg/kg of Aroclor 1254
- Test concentrations with justification for top dose:
- test substance (1st experiment): 5000, 1667, 556, 185 and 62 μg/plate (3 samples)
test substance (2nd experiment): 1667, 556, 185, 62 and 21 μg/plate (3 samples)
control (DMSO): 100 μl (6 samples)
positive control: from 1 to 50 μg/plate, depending on the positive control (3 samples) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The test substance was not enough soluble in water. DMSO is a common
vehicle for the Ames test.
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- TA98, TA100, TA1535, with S9
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Remarks:
- TA97a, with S9
- Positive controls:
- yes
- Positive control substance:
- other: 1,8-Dihydroxy-anthraquinone
- Remarks:
- TA102, with S9
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- TA98, without S9
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA100, TA1535, without S9
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylenediamine
- Remarks:
- TA97a, without S9
- Positive controls:
- yes
- Positive control substance:
- other: t-Butyl-hydroperoxide
- Remarks:
- TA102, without S9
- Untreated negative controls:
- yes
- Remarks:
- Dimethylsulfoxide
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethylsulfoxide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 2 days
NUMBER OF REPLICATIONS: The results were verified by a second, independent experiment. Triplicate repetitions were run for each dose group in each of the two separate experiments that were conducted, for the control groups six-fold repetitions were run.
NUMBER OF CELLS EVALUATED: 2 to 3 x 109 cells per mL
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: A reduced bacterial background lawn (mottled instead of homogeneous), microcolonies of bacteria instead of a homogeneous background lawn, no background lawn, clearly reduced numbers of revertant colonies. - Evaluation criteria:
- Means and standard deviations were calculated for the number of mutants in every concentration group.
The criteria for a positive result are:
A reproducible increase of the number of revertants to more than the following threshold values for at least one of the concentrations:
• For the strains with a low spontaneous revertant rate i.e. TA98 and TA1535: The 2½ fold of the amount of the spontaneous revertants.
• For the strains with a high spontaneous revertant rate i.e. TA97a, TA100 and TA102: The 12/3 fold of the amount of the spontaneous revertants.
These threshold values were derived from the variations in the control samples of the historic data of the Ames test.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA97a, TA98, TA100, TA102 and TA1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Positive controls: All positive control groups showed significantly increased mutation frequencies which demonstrate the sensitivity of the test system.
Test substance:
Toxicity: results of the test substance did not show toxicity up to 1667 μg/plate. At 5000 μg/plate the bacterial background could not be distinguished from the precipitate.
Solubility: a turbidity was visible when the test substance was mixed with the agar at the 5000, 1667 and 556 μg/plate samples. When the colonies were counted, a precipitate impeded the counting of the colonies at 5000 μg/plate. At 1667 and 556 μg/plate a slight precipitate was still observed in the plates with metabolisation, but the colony counting and the determination of toxicity was not affected.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
According to the results obtained in this study, Zirkonium-tetrakis(isobutyrato) is non-mutagenic in the Ames test with the strains TA97a, TA98, TA100, TA102 and TA1535 with and without an external metabolic activation. - Executive summary:
Genetic toxicity: key study in Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535, according to OECD guideline 471 and directive 2000/32/EC, part B.13/14.
Zirkonium-tetrakis(isobutyrato) was tested for mutagenic activity with the "Salmonella typhimurium Reverse Mutation Test" (Ames Test). In the first experiment the following concentrations were tested: 62, 185, 556, 1667 and 5000 μg per plate without external metabolisation, and 62, 185, 556, 1667 and 5000 μg per plate with S9-mix from Aroclor 1254 induced microsomes of rat liver as an external metabolising system. In the second experiment the following concentrations were tested: 21, 62, 185, 556 and 1667 μg per plate without external metabolisation, and 21, 62, 185, 556 and 1667 μg per plate with S9-mix from Aroclor 1254 induced microsomes of rat liver as an external metabolising system. The test was performed according to the "direct plate incorporation method". Negative and positive controls were included. An independent repetition of the experiment was performed.All positive control groups showed significantly increased mutation frequencies which demonstrate the sensitivity of the test system.
Results of the test substance did not show toxicity up to 1667 μg/plate. At 5000 μg/plate the bacterial background could not be distinguished from the precipitate. Solubility: A turbidity was visible when the test substance was mixed with the agar at the 5000, 1667 and 556 μg/plate samples. When the colonies were counted, a precipitate impeded the counting of the colonies at 5000 μg/plate. At 1667 and 556 μg/plate a slight precipitate was still observed in the plates with metabolisation, but the colony counting and the determination of toxicity was not affected.
In none of the concentrations tested and with none of the strains used an increase of the mutation frequency to more than the threshold values was obtained. Metabolic activation did not change these results.
According to the results obtained in this study, Zirkonium-tetrakis(isobutyrato) is non-mutagenic in the Ames test with the strains TA97a, TA98, TA100, TA102 and TA1535 with and without an external metabolising system up to the limit of toxicity.
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