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Diss Factsheets
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EC number: 935-211-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical concentration control
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol
- EC Number:
- 935-211-5
- Cas Number:
- 1305113-15-8
- Molecular formula:
- C30H28O10P2
- IUPAC Name:
- 1,4:3,6-dianhydro-2,5-bis-O-(diphenoxyphosphoryl)-D-glucitol
- Details on test material:
- - Name of test material (as cited in study report): Isosorbid-O,O'-bis(diphenylphosphorsäureester)
- Physical state: solid
- Analytical purity: 97.9 Mol-%
- Storage condition of test material: ambient
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Sampling for concentration control was done within 30 min before insertion of the test organisms (0 h) and after approx. 48 hours and 96 hours from all test vessels. The samples were taken from the middle of the test vessel using a glass pipette and deposited into glass ampoules, which were rinsed with test solution before they were filled. Sample volume approx. 10 mL.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The test substance is poorly soluble in water. Therefore the test solution was prepared using a saturation column in order to achieve a saturated solution of the test substance. The saturation column was prepared by weighing approx. 1.6 g of test substance into a weighing boat and dissolving his mass in approx. 200 mL dichloromethane. The solvent solution was then poured over the pre-cleaned glass wool (approx. 20 g) in a stainless steel pan and the solvent was completely evaporated leaving the test substance adhered to the glass wool. The loaded glass wool was rolled and packed into a glass column (50 cm long with 2.8 cm internal diameter) fitted with a plug of clean glass wool to keep particulate matter in the column. Silicon stoppers fitted with short glass tubes were attached to the ends of the column, which was then connected to a peristaltic pump (Watson Marlow GMBH, Germany, 500 series) via a short length flexible tubing (Watson Marlow GmbH, Germany, Marprene). The packed column was rinsed with dilution water (column flow rate < 1 L/h) for about one day and the rinse water discarded. The test was carried out in a flow-throug system. The outflow of the column was introduced into the 100% test vessels.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Zierfischzüchterei Friedrich, 33739 Bielefeld, Germany
- Age at study initiation: juvenile
- Length at study initiation: 3.0 ± 0.5 cm
- Weight at study initiation: < 3.0 g
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Commercial fish diet, ("Tetramin", Tetra-Werke, Melle, Germany), daily ad libitum, and additionally live juvenile brine shrimp (Artemia nauplii) generally on workdays.
- Medical treatment: none
- Mortality during the last week before start of exposure: < 5%
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 100 CaCO3 mg/L
- Test temperature:
- 23 °C
- pH:
- 7.9 - 8.0
- Dissolved oxygen:
- 8.0 - 8.3 mg/L
- Nominal and measured concentrations:
- Nominal: 0.3 mg/L (and control)
Measured at 0 h: 0.151 mg/L
Measured at 48 h: 0.156 mg/L
Measured at 96 h: 0.1645 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: stainless steel aquaria
- Fill volume: 9 L
- Aeration: none
- Type of flow-through: peristaltic pump
- Renewal rate of test solution (flow rate): at least 2.25 L/h and test vessel, resulting in at least 6 volume exchanges per day
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: max. 1 g fish /L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: charcoal-filtered tap water from the municipal water works of the city of Frankenthal mixed with deionised water
- The tap water used is regularly assayed for chemical contaminants by municipal authorities and BASF SE s well as for presence of microbes by a contract laboratory. On the basis of the analytical findigs, the water was found to be suitable. The German Drinking Water Regulation served as guideline for maximum tolerable contaminant concentrations.
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, temperature and dissolved oxygen daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: ca. 20 - 850 Lux, 400 - 750 nm
EFFECT PARAMETERS MEASURED
Each test vessel was visually checked for mortality and clinical observations (such as abnormal behavior, signs of intoxication and visible morphological alterations) within one hour after start of exposure and after approx. 6, 24, 48, 72 and 96 hours.
TEST CONCENTRATIONS
- Reason for the selection of the test concentrations: In a preliminary test using zebrafish, a nominal concentration of 100 mg/L caused no mortality or signs of toxicity after 96 hours. Since the substance is known to be poorly soluble in water, only a saturated solution was tested.
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.157 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: saturated solution
- Details on results:
- - A saturated solution was tested. No effects were observed within the range of solubility.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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