Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 201-071-2 | CAS number: 77-94-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity to aquatic algae was assessed in a test with Pseudokirchnerella subcapitata (reported as Selenastrum capricornutum) according to OECD 201 (2010):
ErC50 (72h) = 100.4 mg/L, EyC50 (72h) = 23.86 mg/L
NOErC = 6.0 mg/L, NOEyC = 6.0 mg/L
LOErC = 9.7 mg/L, LOEyC = 9.7 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100.4 mg/L
- EC10 or NOEC for freshwater algae:
- 6 mg/L
Additional information
The acute toxicity to aquatic algae and cyanobacteria of tributyl citrate was tested according to OECD guideline 201 and EU Method C.3 in a static dose-response Algal Growth Inhibition Test with the freshwater green algae Pseudokirchneriella subcapitata (formerly known as Selenastrum capricornutum) as test organism (2010). The study was conducted under certificated GLP compliance. The concentrations that have been tested were 4.3, 9.4, 21, 45 and 100 mg test item/L (mean measured) and a control (test water without addition of the test item). For the determination of the test concentrations a range-finding test was performed. However, concentrations above the limit concentration (nominal 100 mg test item/L or limit of solubility) described by the OECD 201 Guideline for Testing of Chemicals, Section 2 from March 2006 were not tested. The study encompassed 6 treatment groups (5 dose rates of the test item, control) with three replicates per test concentration and six replicates for the control. At test start 50 mL of the test concentrations were inoculated with 5000 algal cells per mL test medium. These cells were taken from an exponentially growing pre-culture, which was set up 4 days prior to the test start under the same conditions as in the test. Based on the calculated cell densities, the 72-hour ErC50 and the 72-hour EyC50, the corresponding EC10 values and where possible their 95 %-confidence limits were calculated by Probit analysis. All other calculations were carried out using a statistical software programme. Analysis of the yield and growth rate of the algal population within 72 h exposure period gave the following results:
The 72-hour ErC50 value was calculated to be 100.4 mg test item/L and the 72-hour EyC50 was calculated to be 23.86 mg test item/L. The 72-hour NOErC was determined to be 6.0 and the associated 72-hour LOErC is 9.7 mg test item/L. The 72-hour NOEyC was determined to be 6.0 and the associated 72-hour LOEyC is 9.7 mg test item/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)