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EC number: 225-059-1 | CAS number: 4635-59-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP study; E. coli not tested
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
- Principles of method if other than guideline:
- in accordance with Ames B.N. et al.: Proc. Nat. Acad. Sci. USA, 70, 2281-2285, (1973) as well as Ames B.N. et al.: Mut. Res. 31, 347-364, (1975)
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-chlorobutyryl chloride
- EC Number:
- 225-059-1
- EC Name:
- 4-chlorobutyryl chloride
- Cas Number:
- 4635-59-0
- Molecular formula:
- C4H6Cl2O
- IUPAC Name:
- 4-chlorobutanoyl chloride
- Details on test material:
- Name of the test substance used in the study report: 4-Chlorbuttersäurechlorid
Purity: ca. 99%
Constituent 1
Method
- Target gene:
- S. typhimurium
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9 fraction
- Test concentrations with justification for top dose:
- 20 - 5000 ug/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: with S-9 mix (all strains): 2-Aminoanthracene (10 µg); without S-9 mix: N-Methyl-N'-nitro-N-nitroso-guanidine (5 µg) for strains TA 100, TA 1535 and TA 98, 4-Nitro-o-phenylenediamine (10 µg), 9-Aminoacridinium chloride monohydrate (100 µg) for TA 100
- Details on test system and experimental conditions:
- TEST SYSTEM
System: standard plate test.
Metabolic activation: with and without, using liver S-9 supernatant of male Aroclor 1254 induced Sprague-Dawley
rats.
Incubation: plates were incubated at 37 °C for 48 hours in the dark; the number of colonies was then counted.
Replicates: 4 plates per dose and control were used in 2 independent experiments.
Experiment 1 (standard plate test; with and without S-9 mix;
all strains): 20, 100, 500, 2500 and 5000 µg/plate;
Experiment 2 (standard plate test; with and without S-9 mix;
all strains): 250, 500, 750, and 1000 µg/plate. - Evaluation criteria:
- test results considered positive, if
- doubling of revertant colonies compared to spontaneous mutation rate
- dose effect relationship
- reproducibility of results
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: > 750 ug/plate
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: > 750 ug/plate
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Cytotoxicity:
A bacteriotoxic ffect (reduced his- or trp- background growth, decrease in the number of his+ or trp+ revertants in the titer was observed from about 750 µg onwards
Solubility
No precipitation of the substance was found. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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