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EC number: 204-820-1 | CAS number: 127-06-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- mechanistic studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study meets generally acceptable scientific standards, valid for assessment. No data on GLP.
Data source
Reference
- Reference Type:
- publication
- Title:
- Amination of Tyrosine in Liver Cytosol Protein of Male F344 Rats Treated with 2-Nitropropane, 2-Nitrobutane, 3-Nitropentane, or Acetoxime
- Author:
- Sodum R, Fiala ES
- Year:
- 1 997
- Bibliographic source:
- Chem. Res. Toxicol. 1997, 10, 1420-1426.
- Report date:
- 1997
Materials and methods
- Principles of method if other than guideline:
- In this paper, the authors examined liver proteins of rats treated with acetoxime for the presence of 3-aminotyrosine, the expected product of tyrosine amination. It was used ion-pair and/or cation-exchange high-performance liquid chromatography with electrochemical detection.
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- carcinogenicity
Test material
- Reference substance name:
- Acetone oxime
- EC Number:
- 204-820-1
- EC Name:
- Acetone oxime
- Cas Number:
- 127-06-0
- Molecular formula:
- C3H7NO
- IUPAC Name:
- acetone oxime
- Details on test material:
- - Name of test material (as cited in study report): Acetoxime
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Taconic Labs, Germantown, NY.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- other: Emulphor 620
- Details on exposure:
- Acetoxime was dissolved in an aqueous 10% solution of Emulphor 620 (GAF Corp., Wayne, NJ) for administration to rats.
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- From 6 to 23 h after a single administration.
- Frequency of treatment:
- Only one administration.
- Post exposure period:
- Rats were sacrificed 6, 18, or 23 h after administration.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
3.3 mmol/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- One male rat per time point.
- Control animals:
- yes, concurrent vehicle
- Details on study design:
- HPLC Instrumentation:
Solvent was delivered with the Shimadzu model LC-600 HPLC pumps (Shimadzu Scientific Instruments, Braintree, MA) with Shimadzu model SCL-6B solvent programmer. The eluate was analyzed using the Waters model 996 photodiode array detector and the BAS (Bioanalytical
Systems, West Lafaytte, IN) model LC-4B amperometric detector with a glassy carbon working electrode and the Ag/AgCl/3 M NaCl reference electrode. An electrode potential of 475 mV was used for analysis of protein hydrolysates and 600 mV was used for nucleoside analysis. Data were collected and processed using the Waters Millennium Chromatography Manager.
Protein Sample Preparation:
Rat livers were thawed at 4 °C and homogenized, and the cytosol supernatant was obtained by centrifugation at 107000 g. Protein concentrations in the cytosolic fractions were 25 mg/mL as determined using the Sigma protein determination kit. Protein was obtained from the cytosolic fractions by precipitation with excess acetone containing 0.2% HCl and was then dried under nitrogen. The protein was hydrolyzed under vacuum in protein hydrolysis tubes with 6 N HCl at 150 °C for 1 h; then the HCl was removed by evaporation under a stream of nitrogen. Under these hydrolysis conditions 85% of added 3-aminotyrosine could be recovered. The dry residue was redissolved in 1-2 mM sodium citrate, pH 3.5, and analyzed by ion-pair and cation-exchange HPLC with UV and EC detectors.
Examinations
- Examinations:
- Rats were sacrificed, and the livers were removed and frozen immediately in liquid nitrogen before storage at -80 ºC.
Results and discussion
- Details on results:
- It was found that the liver cytosolic proteins of these animals contained 0.53 ± 0.03 mol of 3-aminotyrosine/1E+03 mol of tyrosine.
Any other information on results incl. tables
It was found that the liver cytosolic proteins of these animals contained 0.53 ± 0.03 mol of 3-aminotyrosine/1E+03 mol of tyrosine.
Applicant's summary and conclusion
- Conclusions:
- It was found that the liver cytosolic proteins of these animals contained 0.53 ± 0.03 mol of 3-aminotyrosine/1E+03 mol of tyrosine.
- Executive summary:
In this paper, the authors examined liver proteins of rats treated with acetoxime for the presence of 3-aminotyrosine, the expected product of tyrosine amination.
Using ion-pair and/or cation-exchange high-performance liquid chromatography with electrochemical detection, it was found that the liver cytosolic proteins of these animals contained 0.53 ± 0.03 mol of 3-aminotyrosine/1E+03 mol of tyrosine.
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