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EC number: 606-946-6 | CAS number: 221640-14-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Apr - Jun 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 23.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 11.6 - 49.1
- Validity criteria fulfilled:
- yes
- Conclusions:
- The ECso/48 hours ofZK 155546 to Daphnia magna was calculated to be 23.9 mg/L [95% confidence limits 11.6 - 49.1] on the basis of TOC
measured concentrations. The highest concentration without effects was 7.0 mg/L.
Accordingly, ZK 155546 is harmful to Daphnia magna, since the EC50 was between 10 and 100 mg/L. - Executive summary:
The purpose ofthe study was to determine the acute toxicity (immobilization, EC50/48 hours) of Z-Triamin-Dihydrochlorid (ZK 155546) with Daphnia magna in order to evaluate the aquatic toxicity. ZK 155546 is an intermediate of the synthesis of Primovist. The study was conducted as a definitive test according to the guideline of the OECD, no 202.
20 juvenile Daphnia magna were used for each test solution of ZK 155546 and 20 for the tap water control. The Daphnia were exposed for aperiod of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation ofthe test solutions a suspension with a nominalloading of 111 mg/L was ultrasonified and then stirred for approximately 30 minutes each. This suspension was filtered through a glassfibre filter. The resulting solution was used for the preparation of the test solutions. The highest concentration had a nominal concentration of 100 mg/L obtained by dilution with tap water containing the daphnids, the further concentrations were obtained by dilutions of 1 :2, 1 :4, 1 :8, and 1 :16 with tap water. Additionally, a control solution was prepared with tap water without test material. Sampies for the concentration analysis by TOC were taken at 0 and 48 hours after start of exposure. The analytical results were in good agreement with the nominal concentrations. Statistical evaluation regarding the observations of immobilization was perfonned by pro bit analysis.
There was a clear concentration-effect relationship from 13.7 mg/L upwards at 24 and 48 hours. Thus, the immobilized Daphnia at 13.7 mg/L are assumed to be substance related, even if the accepted spontaneous immobilization rate of 10 % for the controls by the OECD guideline is considered. 100% of the Daphnia were immobilized at 22.5 mg/L ZK 155546 after 48 hours.
Reference
Table 1: Cumulative immobilization (%) of Daphnia magna (0 = 20) after exposure to ZK 155546
Nominal concentration | Measured concentration of | 24 hours | 48 hours |
of ZK 155546 (mg/L) | ZK 155546 (mg/L) | ||
0 control (tap water) | 0 control (tap water) | 0 | 0 |
6.25 | 7.0 0 0 | 0 | 0 |
12.5 | 13.7 | 5 | 10 |
25 | 22.5 | 10 | 100 |
50 | 50.2 | 85 | 100 |
100 | 100.7 | 100 | 100 |
Description of key information
The ECso/48 hours ofZK 155546 to Daphnia magna was calculated to be 23.9 mg/L [95% confidence limits 11.6 - 49.1] on the basis of TOC
measured concentrations. The highest concentration without effects was 7.0 mg/L.
Accordingly, ZK 155546 is harmful to Daphnia magna, since the EC50 was between 10 and 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 23.9 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.