Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 476-280-7 | CAS number: 109113-72-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-01-29 to 2007-03-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Version / remarks:
- 1996
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: samples from the test media of the test concentration and the control at the start and at the end of the test
- Sampling method: samples were taken from the approximate centre of the aquaria without mixing of the test media
- Sample storage conditions before analysis: All samples were analysed immediately after sampling. The samples are kept stored in deep-freezer up to the date of the final report. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
A stock solution with a concentration of 400 mg/L was prepared with test item and ISO-medium. At this concentration the test item formed a stable dispersion (suspension) in the medium used. The work solution was prepared by diluting the stock solution by a 400-fold dilution (2.5 ml ad 1000 ml ISO-medium). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Daphnia magna (Straus), Female
- Source: National Institute of Public Health, Hungary
- Age at study initiation (mean and range, SD): Less than 24 hours old
- Method of breeding: They were bred in the laboratories of LAB International Hungary under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding to pH, components of the main ions and total hardness as the test water used in the test.
- Feeding during test: None
ACCLIMATION
- Acclimation period: > 6 hours
- Acclimation conditions (same as test or not): same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 168.0 mg CaCO3/L (Reconstituted Water)
- Test temperature:
- 20.7 – 21.3 °C
- pH:
- 6.87 - 7.41
- Dissolved oxygen:
- 6.2 - 6.9 mg/L
- Nominal and measured concentrations:
- - Nominal: 0.063; 0.125; 0.250; 0.50 and 1.00 mg test item/L
- Measured: 0.046; 0.108; 0.210; 0.375; and 0.780 mg test item/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Material, fill volume: 40 mL test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The ISO medium (according to OECD 202) was prepared by adding 25 ml each of the following stock solutions to deionised water.
CaCl2 x 2 H2O 11.76 g/L; Batch No.: 828771, Supplier: REANAL
MgSO4 x 7H2O 4.93 g/L; Batch No.: A677186 601, Supplier: MERCK
NaHCO3 2.59 g/L; Batch No.: 829880, Supplier: REANAL
KCl 0.23 g/L; Batch No.: KBM5010801, Supplier: REANAL
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, pH, Dissolved Oxygen and Water Temperature
TEST CONDITIONS:
- Observation time: 24 and 48 hours after start of the test - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.57 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Remarks on result:
- other: 95% CL = 0.46 - 0.71
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL = 0.16 – 0.21
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.78 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: nominal: 1.00
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- < 0.046 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: nominal: 0.063
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 0.046 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: nominal: 0.063
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 value of 0.18 mg/L at 21°C for Daphnia magna was determined for the test item under the conditions of the test. It was determined in a reliable study conducted according to appropriate standard test methods (EU Method C.2 (Acute Toxicity for Daphnia)/OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test)).
Reference
Analytical results:
The analytically determined mean test item concentrations in the test media varied in the range of 56 % to 114 % of the nominal value.
Concentration (mg/L) | %-loss in the measured test item concentration during the test | |||
nominal | measured | |||
start | end | average | ||
0.063 | 0.055 | 0.036 | 0.046 | 34.55 |
0.125 | 0.142 | 0.073 | 0.108 | 48.59 |
0.250 | 0.28 | 0.14 | 0.210 | 50.00 |
0.500 | 0.46 | 0.29 | 0.375 | 36.96 |
1.000 | 0.92 | 0.64 | 0.780 | 30.43 |
Description of key information
A 48 -h EC50 value of 0.18 mg/L for Daphnia magna was determined for the test item under the conditions of the test. It was determined in a reliable study conducted according to an appropriate standard test method (OECD 202).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.18 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.