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EC number: 219-606-3 | CAS number: 2478-10-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Jan 1990 to 18 Jan 1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- no analytical monitoring
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- June 1984
- Deviations:
- no
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Butanediolmonoacrylate
- Appearance: clolourless, light yellowish
- Water solubility: miscible in any ratio
- Stability: polymerisation >25 °C
- Storage: 6 months - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Preparation of stock solution: The stock solution (concentration of 84.0 mg/L) was prepared in a volumetric flask with bidest. water. Solvents were not used. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Algae collection Goettingen Continuous Culture
- Source: Oekolimna
- Age of inoculum (at test initiation): 3 days
- pH of culture medium: 8.2 ± 0.3
- Culturing volume: 100 mL
- Temperature: 20 °C
- Stock culture was renewed once a week. Alge were purchased in agar tubes from the university of Göttingen. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20 ± 1.0 °C
- pH:
- 8.1 - 8.9
- Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control), 4.0, 6.4, 10.2, 16.4, 26.2, and 42.0 mg/L
- Measured test concentrations: not determined. - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass reaction tube
- Fill volume: 10 mL test media
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
GROWTH MEDIUM
- Standard medium used: yes, according to DIN 38412, part 9
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Photoperiod: 24 h light per day
- Light intensity and quality: 10000 lux, 0.72 E+20 photons/m² x s
EFFECT PARAMETERS MEASURED
Algal cell concentrations were determined after 0, 24, 48 and 72 hours, by measuring fluoerescence (with a Flourimeter).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6 - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.61 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 4.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 44.61 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The inhibition of growth rate increased in a dose-dependent manner from 9.25% at the lowest test concentration (4.0 mg/L) up to 98.64% inhibition at 42 mg/L. Inhibition of biomass was 33.9% at 4.0 mg/L and increased in a dose-dependent manner up to 100% inhibition at 42 mg/L. For an overview of the results, see 'Any other information on materials and methods incl. tables'.
- Reported statistics and error estimates:
- Effect concentrations were estimated graphically and calculated by Probit analysis.
- Validity criteria fulfilled:
- not specified
Reference
Table: inhibition of algal growth
Test concentration |
Biomass |
Growth rate |
Inhibition in %, biomass |
Inhibition in %, growth rate |
mg/L |
B |
µ |
HB |
Hµ |
0 (control) |
1.37 |
1.09 |
|
|
4.0 |
0.91 |
0.99 |
33.94 |
9.25 |
6.4 |
0.59 |
0.88 |
56.84 |
19.27 |
10.2 |
0.26 |
0.65 |
80.75 |
40.34 |
16.4 |
0.12 |
0.45 |
91.06 |
58.76 |
26.2 |
0.04 |
0.27 |
97.08 |
74.92 |
42.0 |
-0.01 |
0.01 |
100 |
98.64 |
ADDITIONAL EFFECT VALUES
EbC10 (72 h) = 2.17 mg/L
EbC50 (72 h) = 5.49 mg/L
EbC90 (72 h) = 13.96 mg/L
Description of key information
ErC50 = 13.6 mg/L (Desmodesmus subspicatus)
ErC10 = 4.16 mg/L (Desmodesmus subspicatus)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 13.6 mg/L
- EC10 or NOEC for freshwater algae:
- 4.16 mg/L
Additional information
The toxicity towards freshwater algae was determined in a study according to OECD TG 201 (Oekolimna, 1990). In this study, exponentially growing freshwater alga (Desmodesmus subspicatus) were exposed to nominal test substance concentrations of 0 (control), 4.0, 6.4, 10.2, 16.4, 26.2, and 42.0 mg/L for 72 hours under static conditions. Test concentrations were not analytically verified. At the start of the test and after 24, 48 and 72 hours exposure duration, algal cell concentrations were determined by measuring fluorescence. The inhibition of growth rate increased in a dose-dependent manner from 9.25% at the lowest test concentration (4.0 mg/L) up to 98.64% inhibition at 42 mg/L. Inhibition of biomass was 33.9% at 4.0 mg/L and increased in a dose-dependent manner up to 100% inhibition at 42 mg/L. Based on these findings, the 72-h EC50 values for growth rate and biomass are determined at 13.61 mg/L and 5.49 mg/L, respectively. The 72-h EC10 values for growth rate and biomass are 4.16 mg/L and 2.17 mg/L, respectively.
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