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EC number: 615-497-5 | CAS number: 71809-65-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 32085-79-3
- EC Number:
- 608-700-3
- Cas Number:
- 32085-79-3
- IUPAC Name:
- 32085-79-3
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical verification of the test item concentrations was carried out after 0 and 48 hours. For stability control a series of the concentrations
tested, was made up in the same way as the concentrations for biological part, but in separate vessels without test organisms.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Stock solution: For the preparation of a stock solution, 10 g/L of the test item was equilibrated in completely desalted water. The resulting solution
was used as stock solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name: Daphnia magna Straus, clone 5
Origin: The water flea Daphnia magna Strauss used is that of clone 5. Originally, the animals were received 1991 from Dr. N. Caspers (Bayer AG,
Leverkusen, Germany); subsequently they were bred in house. The operating steps necessary to breed the animals are given in a Standard
OperatingProcedure.
Culture conditions: For breeding, the females are maintained in M4 medium in 1 L beakers. The daily amount of food, comprising Desmodesmus
subspicatus, is such that the amount added is consumed by the animals. A reddish dark-brown coloration of the animals indicates that they are
healthy. At two to three day intervals, the exuviae are syphoned off and the water is changed. ln the course of this, the offspring is removed from thebreeding vessels. At regular intervals of approximately 4 weeks, offspring is isolated from the vessels. They serve as a basis for further breeding.
The culture conditions ensure parthenogenetic reproduction.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 14°dH = 250 mg CaCO3/L
- Test temperature:
- 20.0-20.3°C
- pH:
- 7.7-9.5
- Dissolved oxygen:
- 7.5-9.1 mg/L
- Nominal and measured concentrations:
- nominal 250, 350, 500, 700and 1000 mg/L
- Details on test conditions:
- Test vessels: Round-bottom test tubes calibrated to 10 ml
Experimental set-up: 5 test item concentrations + 1 control, 20 organisms in 4 parallel sets of 5 each
Density of occupation of animals: ca. 2 ml per test organism
Light/dark cycle: dark
Temperature: 20.0- 20.3°C (mean: 20.2°C)
Aeration: none
Feeding: none
Solubilizer: none - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The highest concentration tested in this experiment at which after 48 h exposure no immobilisation (<=10%) occurred, was 1000 mg/L.
The lowest concentration tested in this experiment at which after 48 h exposure 100 % immobilisation occurred, was >1000 mg/L. - Results with reference substance (positive control):
- Daphnia magna. lnfluence of potassium dichromate on the swimming behaviour after 24 h exposure.
concentration (mg/ L) % of immobilised Daphnia
1.0 20
2.0 100
The results demonstrate that the sensitivity of the individuals lies in the normal range.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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