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EC number: 418-310-3 | CAS number: 126050-54-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 3 April 1989 to 18 May 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: "Standards to be observed by Mutagenicity Testing Institutions" issued by the Japanese Ministry of Labour,
- Principles of method if other than guideline:
- The study was conducted in compliance with "Good Laboratory Practice (GLP) Standards as set forth in Article 4 relating to Test Items of New Chemical Substances and Survey Items of Toxicity of Designated Chemical Substances'' issued by the Japanese Ministry of International Trade and Industry (MITI) etc. and "Standards to be observed by Mutagenicity Testing Institutions" issued by the Japanese Ministry of Labour, and the report presents a full and accurate account of the results of the study.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Name of the new chemical substance: 2,4,8,10-Tetrakis(1,1-dimethyl ethyl)-6-(2-ethylhexyloxy)-12H-dibenzo [d,g] [1,3,2]dioxaphosphocin
Other name: 2,2'-Methylenebis(4.6-di-tert-butylphenyl)2-ethylhexyl phosphate
Lot No. (Batch No.): 102
Purity of the new chemical substance tested: 100 %
Molecular weight: 583
Appearance at ordinary temperature: White powder
Stability
Heat: Stable below 200'C
Light: Stable
Water: Stable(until 10 days)
DMSO: Stable(until 10 days)
Acetone: Stable(until 10 days)
Degree of solubility
Water: Less soluble
DMSO: 0.8%
Acetone: 1.3%
Method
- Target gene:
- Histidene
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Dose determination test: 1, 5, 10, 50, 100, 500, 1000, 5000 μg/plate
Main test: 156, 313, 625, 1250, 2500, 5000 μg/plate - Vehicle / solvent:
- Dimethylsulfoxide (DMSO)
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2- (2- Fury I)-3- (5-nitro-2-furyl)- acrylamide (AF- 2); 2- Aminoanthracene ( 2- AA)
- Details on test system and experimental conditions:
- Test substance: 2,4,8,10-Tetrakis (1,1-dimethyl ethyl)-6-(2-ethylhexyloxy)-12H-dibenzo[d.g] [1,3,2] dioxaphosphocin [other name: 2,2'-methylenebis(4,6-di-tertbutylphenyl)2-ethylhexyl phosphite] was suspended in dimethylsulfoxide (DMSO) by uitrasonification.
Bacterial strains: Salmonella typhimurium stranis TA98, TA100. TA1535 and TA1537 and Escherichia coli WP2uvrA were supplied by Dr. T. Matsushima, Department of Molecular Oncology, Institute of Medical Science, University of Tokyo.
Bacterial cultures in Oxoid nutrient broth #2 were freshly prepared before use by inoculating bacteria from frozen stock cultures (kept at -80°C).
Fifty μl stock solution was inoculated into the 15ml broth and incubated for 10 hours at 37°C and 180rpm using rotary shaker.
S9 and S9Mix;: S9, post-mitochondrial supernatant of rat-liver homogenates was obtained from Kikkoman Corporation. Chiba (JAPAN). This S9 was prepared by slight modifications of method of Ames [1] :5.6-Benzoflavone and Phenobarbital were used as inducers of drug-metabolizing enzyme system [2]. The S9mix contained 4mM NADPH, 4mM NADH. SmM G-6-P. 8mM MgCl2, 33mM KCI. 100mM sodium phosphate buffer (pH 7.4) and 10% S9 (50 μl S9 per plate). - Evaluation criteria:
- Not specified in the study report.
- Statistics:
- Not specified in the study report.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- 2,4,8,10-Tetrakis(1,1-dimethyl ethyl)-6-(2-ethylhexyloxy)-12H-dibenzo[d,g] [1 ,3,2] dioxaphosphocin did not induce revertant colonies more than twice of that of the solvent control on the S. typhimurium TA98, TA100, TA1535 and TA1537, and E. coli WP2uvrA with or without metabolic activation.
Positive control substances showed increases in revertant colony count more than twice of the solvent control values with bacterial strains in various tests, and these suggested that susceptibilities of test bacterial strains were adequately maintained and the test was adequately carried out.
Based on the above results, the present test substance was assessed to be negative. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Based on the results, the present test substance was assessed to be negative. - Executive summary:
Purpose of this test is an evaluation of the mutagenicity of 2,4,8,10-tetrakis(1,1-dimethyl ethyl)-6-(2-ethylhexyloxy)-12H-dibenzo [d.g] [1,3,2]dioxaphosphocin [other name: 2,2'-Methylenebis(4,6-di-tert-butylphenyl)2-ethylhexyl phosphite] by the microbial mutagenicity test.
The study was conducted in compliance with "Good Laboratory Practice (GLP) Standards as set forth in Article 4 relating to Test Items of New Chemical Substances and Survey Items of Toxicity of Designated Chemical Substances'' issued by the Japanese Ministry of International Trade and Industry (MITI) etc. and "Standards to be observed by Mutagenicity Testing Institutions" issued by the Japanese Ministry of Labour, and the report presents a full and accurate account of the results of the study.
2,4,8,10-Tetrakis(1,1-dimethyl ethyl)-6-(2-ethylhexyloxy)-12H-dibenzo[d,g] [1,3,2] dioxaphosphocin did not induce revertant colonies morethan twice of that of the solvent control on the S. typhimurium TA98,TA100, TA1535 and TA1537, and E. coli WP2uvrA with or without metabolic activation.
Positive control substances showed increases in revertant colony countmore than twice of the solvent control values with bacterial strains in various tests, and these suggested that susceptibilities of test bacterial strains were adequately maintained and the test was adequately carried out.
Based on the above results, the present test substance was assessed to be negative.
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