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EC number: 243-497-1 | CAS number: 20073-13-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was performed between 09 September and 16 September 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl [1α,2β(Z)]-(±)-3-oxo-2-(pent-2-enyl)cyclopentaneacetate
- EC Number:
- 243-497-1
- EC Name:
- Methyl [1α,2β(Z)]-(±)-3-oxo-2-(pent-2-enyl)cyclopentaneacetate
- Cas Number:
- 20073-13-6
- Molecular formula:
- C13H20O3
- IUPAC Name:
- methyl 2-[(1S,2S)-3-oxo-2-[(2Z)-pent-2-en-1-yl]cyclopentyl]acetate
Constituent 1
- Specific details on test material used for the study:
- Sponsor's identification: JASMONEIGE (Lot No. 75028)
Lot number: 75028
Date received: 24 August 1999
Description: colourless liquid
Storage conditions: approximately 4° C in the dark under nitrogen
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Three New Zealand White rabbits supplied by David Percival Ltd, Moston, Sandbach, Cheshire, UK were used. At the start of the study the animals weighed 2.41 to 2.66 kg and were twelve to sixteen weeks old. After a minimum acclimatisation period of five days each animal was given a number unique within the study which was written with a black indelible marker-pen on the inner surface of the ear and on the cage label.
The animals were individually housed in suspended metal cages. Free access to mains drinking water and food (STANRAB SQC Rabbit Diet, Special Diets Services Ltd, Witham, Essex, UK) was allowed throughout the study.
The temperature and relative humidity were controlled to remain within target ranges of 17 to 23 °C and 30 to 70% respectively. Occasional deviations from these targets were considered not to have affected the purpose or integrity of the study. The rate of air exchange was approximately fifteen changes per hour and the lighting was controlled by a time switch to give twelve hours continuous light and twelve hours darkness.
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- 0.5 mL of test item
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 7 days
- Number of animals:
- 3
- Details on study design:
- Procedure
On the day before the test each rabbit was clipped free of fur from the dorsal flank are using veterinary clippers. Only animals with a healthy intact epidermis by gross observation were selected for the study.
On the day of the test a suitable site was selected on the back of each rabbit. A quantity of 0.5 mL of the test material was introduced under a 2.5 cm x 2.5 cm cotton gause patch and placed in position on the shorn skin. The patch was secured in position with a strip of surgical adhesive tape (BLENDERM: approximate sice 2.5 cm x 4.0 cm). To prevent the animals interfering with the patches, the trunk of each rabbit was wrapped in a an elasticated corset (TUBIGIP) and the animals were returned to their cages for the duration of the exposure period.
Four hours after application the corest and patches were removeed from each animal and any residual test material removed by gentle swabbing with cotton wool soaked in 74 % Industrial Methylated Spirits.
Approximate one hour following the removal of the patches, and 24, 48 and 72 hours later, the test sites were examined for evidence of primary irritaion and scored according to the following scale:
EVALUATION OF SKIN REACTIONS
Erythema and Eschar Formation Value
No erythema 0
Very slight erythema (barely perceptible) 1
Well-defined erythema 2
Moderate to severe erythema 3
Severe erythema (beet redness) to slight
eschar formation (injuries in depth) 4
Oedema Formation Value
No oedema 0
Very slight oedema (barely perceptible) 1
Slight oedema (edges of area well-defined by definite 2
raising)
Moderate oedema (raised approximately 1 millimetre) 3
Severe oedema (raised more than 1 millimetre and extending
beyond the area of exposure) 4
Any other skin reactions, if present, were also recorded.
An additional observation was made on Days 7 to assess the reversibility of skin reactions.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible
- Irritant / corrosive response data:
- Very slight erythema was noted at all treated skin sites one hour after patch removal. Well defined erythema was noted at two treated skin sites with very slight erythema at the remaining treated skin site at the 24 hour observation. Well defined erythema persisted at one treated skin site with very slight erythema at two treated skin site with very sligth erythema at two treated skin sites at the 48 hours observation. very slight erythema was noted at one treated skin site at the 72 hours observation.
Very slight oedema was noted at all treated skin sites at the 24 hours observation and persisted at one treated skin site at the 48 hour observation.
Two treated skin sites appeared normal at the 72 hour observations and the remaining treated skin site appeared normal at the 7 day observation.
Any other information on results incl. tables
Individual daily and mean scores for dermal irritation following 4 hour exposure
Skin Reaction | Reading (Hours) | Individual Scores - Rabbit Number and Sex (Bodyweight kg) |
||
35 Male (2.41) |
36 Male (2.45) |
38 Male (2.66) |
||
Erythema/Eschar Formation | 24 | 1 | 2 | 2 |
48 | 1 | 1 | 2 | |
72 | 0 | 0 | 1 | |
Total | 2 | 3 | 5 | |
Mean Score | 0.7 | 1.0 | 1.7 | |
Oedema Formation | 24 | 1 | 1 | 1 |
48 | 0 | 0 | 1 | |
72 | 0 | 0 | 0 | |
Total | 0 | 1 | 2 | |
Mean Score | 0. | 0.3 | 0.7 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test materials did not meet the criteria for classification as irritant or corrosive according to CLP.
- Executive summary:
A study was performed to assess the irritancy potential of the test material to the skin of New Zealand White rabbit. The method used followed the recommendations of the OECD Guidelines for Testing of Chemicals No. 404 "Acute Dermal Irritation/Corrosion" (adpoted 17 July 1992) and Methods B4 of Commission Directive 92/69/EEC.
A single 4 hours, semi-occluded application of the test material to the intact skin of three rabbits produced very slight to well defined erythema and very slight oedema. Two treated skin sites appeared normal at th 72 hours observation and the remaining treate skin site appeared normal at the 7 day observation.
The test material did not meet the criteria for classification as irritant or corrosive according to CLP.
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