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EC number: 427-370-1 | CAS number: 164288-56-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline, GLP study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- a stock solution (1000 mg of test substance a.i - per litre) was prepared 1 hour (approx.) before the start of the exposure, dissolving the test substance into dilution water ; pH of this stock solution was not adjusted.
- Vehicle:
- yes
- Test organisms (species):
- activated sludge
- Details on inoculum:
- • the sludge was used on the day of collection.
• the sludge was mixed during 1 minute in order to destroy the coarse particles.
• the concentration of suspended solids was evaluated from a small amount of sludge.
• the sludge was centrifuged and the suspended solids obtained were washed before to be resuspended for the preparation of the inoculum (centrifuged 3 times with demineralized water containing synthetic sewage for 5 minutes at 2000 r.p.m., with discarding of the supernatants)
• the suspended solids were then re-suspended into synthetic sewage so as to obtain a nominal concentration of suspended solids of approximately 4 grammes per litre in the inoculum
• this inoculum was kept aerated and at thermoregulated temperature (20°C ± 2°C) until its using for the preparation of the media.
Source: activated sludge from a sewage treatment plant treating predominantly domestic sewage (La Fouillouse (Loire) - France). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- ranged from 20.0 °c to 24.1 °c
- pH:
- pH of the test media at the start of the study: ranged from 7.23 to 7.42
pH of the test media at the end of the study: ranged from 7.78 to 8.09 - Dissolved oxygen:
- Aeration: continuous aeration (air-flow : a rate of 10 to 20 bubbles per second approximately) and continuous magnetic stirring.
- Nominal and measured concentrations:
- 12, 27, 61, 135, and 300 mg
- Details on test conditions:
- Sludge nominal concentration: 1,600 mg of suspended solids per litre of control, reference and test media.
Test volumes: 500 mL per flask
Containers: 1,000 mL beakers with covers
Measurements of the dissolved oxygen concentration over a period of 10 minutes (or until the concentration reaches 1 mg/l) after approximately 3 hours exposure, in 300 ml samples from each container.
These measurements were performed in appropriate BOD-flasks, with O2-electrode. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: O2 consumption
- Validity criteria fulfilled:
- yes
- Conclusions:
- Taking into account the results obtained under the experimental conditions employed, these ones may be expressed as follows:
EC 50-3 hours: 64mg/L (a.i.) - Executive summary:
Compliance with OECD Guideline No. 209 (1984) and EEC Directive 88/302.L133-Part C (1988).
METHOD: Micro-organisms from activated sludge (1,600 mg of suspended solids per litre of medium) were exposed to a range of concentrations of test substance for 3 hours 12, 27, 61, 135, and 300 mg test substance (active matter) per litre of test medium,
- Two control media (without test substance) were tested, one at the beginning of the study, the other one at the end of the study.
- The dissolved oxygen concentration was measured in 300 mL samples from each medium after approximately 3 hours of exposure.
- A reference substance, 3,5 - dichiorophenol, was tested under the same conditions in order to control the sensitivity of the activated sludge (3 concentrations).
- The criteria of quality for the study, as notified in the OECD and EEC standards and relative to the level of oxygen consumption in the two control media and to the toxicity of 3,5 - dichlorophenol, were respected.
RESULTS: Taking into account the results obtained under the experimental conditions employed, these ones may be expressed as follows:
EC 50-3 hours: 64mg/L(active mater)
Reference
Description of key information
Taking into account the results obtained under the experimental conditions employed, these ones may be expressed as follows:
EC 50-3 hours: 64mg/L (a.i.)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 64 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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