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EC number: 434-770-8 | CAS number: 55314-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- August 31, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 434-770-8
- EC Name:
- -
- Cas Number:
- 55314-16-4
- Molecular formula:
- C10 H12 N2 O1
- IUPAC Name:
- 3-(dimethylamino)-1-(pyridin-3-yl)prop-2-en-1-one
- Details on test material:
- Name of test material (as cited in study report): STI571 Y5a
- Substance type: Brown solid
- Physical state: Solid
- Analytical purity: 98.6%
- Lot/batch No.: 992301
- Expiration date of the lot/batch: 01 July 2001
- Stability under storage conditions: Stable
- Storage condition of test material: In refrigerator in the dark
- Solubility in water: Not indicated
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Since STI571 Y5A is well soluble in water a stock solution of 5 g/l was prepared in milli-Q water.
Thorough mixing was used to accelerate dissolving and to ensure homogeneity.
The pH of the clear orange, slightly foaming, stock solution was 10.3.
Five concentrations were tested: 100, 180, 320, 560 and 1000 mg/l, corresponding to 10, 18, 32, 56 and 100 ml of the prepared stock solution in 500 ml final volume
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge was coarsely sieved, washed and diluted with tap water. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (3.2 g/l of sludge). Before use the pH was checked (measured value: 7.8). The batch of sludge was used on subsequent days (maximum four days). Therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of each working day. The sludge was kept aerated at test temperature until use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- Oxygen consumption was measured and recorded for approximately 10 minutes after the 3 hours exposure period
Test conditions
- Test temperature:
- The temperature of the test medium was 20°C.
- pH:
- Between 7.7 and 7.8
- Dissolved oxygen:
- Oxygen consumption:
Controls reference substance series: 54-56 mg/ O2/l/h
Reference substance 3, 5 Dichlorophenol:9-51 mg O2/l/h
Controls STI571 Y5A series:50-56 mg O2/l/h
STI571 Y5A Series:45-60 mg O2/l/h
The oxygen concentration at the start of measurement was at least 6.6 mg O2/l - Nominal and measured concentrations:
- Nominal concentration: 0, 100, 180, 320, 560 and 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: All glass, approximately 300 ml oxygen bottles (measuring) and
500 litre test bottles(exposure)
- Aeration:During exposure with clean, oil-free air
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):2/ series
- Biomass loading rate:1.28 g/l suspended solids in final test mixture
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis (Milli-RO
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rate from each vessel, in mg O2/l/hr, was calculated from the linear part of the respiration curve, which was generally between 2.5 and 6.5 mg O2/l.
TEST CONCENTRATIONS
- Test concentrations:0, 100, 180, 320, 560 and 1000 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No inhibition in respiration rate of the sludge was recorded at 100 and 180 mg STI571 Y5A per litre. At higher concentrations very slight inhibition in respiration rate of the sludge was recorded.
No dose dependent increase in inhibition was observed at the three highest concentrations tested. Inhibition in respiration rate of the sludge levelled-off at approximately 15%, which was reached at exposure to 320 mg/l and higher. Hence, the EC50 for STI571 Y5A exceeded 1000 mg/l, the highest concentration tested. - Results with reference substance (positive control):
- Results with reference substance valid: Yes
The inhibitory effect of 3,5-dichlorophenol on aerobic waste water (activated sludge) bacteria increased with increased with increasing concentration , ranging from a 31 % inhibition at 3.2 mg/l to 84% at 32 mg/l.
Relevant effects levels: The EC50 of 3,5-dichlorophenol was: 7.3 mg/l
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Although STI571 Y5A was slightly toxic to waste water (activated sludge) bacteria in this present test, the EC50-value was not reached up to 1000 mg/l. The inhibitory effect of STI571 Y5A on aerobic waste water (activated sludge) bacteria appeared to level off at approximately 15%, which was reached at 320 mg/l and higher.
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