SDBR

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1992

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Samples (n=2) of the loading rate and the control taken at t=0h (newly prepared media) and t=24h (spent media) were analysed.

Vehicle:

no

Details on test solutions:

A quantity of 100 mg of the test substance was accurately weighed out and mixed with one liter of DSWL-E in a glass Erlenmeyer flask with a tap at the bottom, followed by 20h mechanical stirring and 4h standing. Then the medium was tapped off (the first 200 mL was discarded).
The test was performed in 150 mL all-glass beakers, each containing 100 mL of exposure medium. Four beakers containing 5 Daphnia's each were used for each exposure medium.
The exposure media were not aerated and replaced after 24h. The daphnia's were not fed during the test. The pH and oxygen concentration were also measured after 24 hours in the spent and in the clean exposure media.

Test organisms (species):

Daphnia magna

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

ca 210 mg CaCO3/L

Test temperature:

20.1 - 20.4°C

pH:

8.1 - 8.3

Dissolved oxygen:

= 8.1 mg O2/L

Nominal and measured concentrations:

Nominal concentrations: 0 and 100 mg/L.
Measured concentrations at t=0h: 0 and 0.084 mg/L.
Measured concentrations at t=24h: 0 and 0.048 mg/L.

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The average concentration at the WSF at t=0h and t=24h were 0.084 and 0.048 mg/L, respectively.
No mortality was observed in any of the groups. Condition of all test animals was equal to that of the control animals.

All media were completely clear (visually assessed) throughout the test.

There was no immobility in the control. The dissolved oxygen concentration was above 60% of the air saturation value.

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of SDBR to Daphnia magna was determined to be above the water solubility limit (nominal: 100 mg/L; measured 0.048 mg/L).

Description of key information

The acute toxicity of SDBR to Daphnia magna was determined to be above the water solubility limit (nominal: 100 mg/L; measured 0.048 mg/L).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L