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EC number: 236-124-9 | CAS number: 13177-41-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- (1992)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Version / remarks:
- (2008)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- LAUS GmbH, Auf der Schafweide 20, D-67489 Kirrweiler (Germany)
Test material
- Reference substance name:
- Dimethyloctadecyl(3-sulphopropyl)ammonium hydroxide
- EC Number:
- 236-124-9
- EC Name:
- Dimethyloctadecyl(3-sulphopropyl)ammonium hydroxide
- Cas Number:
- 13177-41-8
- Molecular formula:
- C23H49NO3S
- IUPAC Name:
- dimethyl(octadecyl)(3-sulfopropyl)azanium hydroxide
- Test material form:
- solid
1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a biologic sewage treatment plant (NW-Lachen-Speyerdorf, Germany) was used as inoculum. The sewage treatment plant is treating mostly domestic sewage
- Laboratory culture: no
- Preparation of inoculum for exposure: The sludge was filtered, washed with tap water twice, then washed with and re-suspended in test medium. The activated sludge was then aerated until use.
- Pretreatment: no
- Concentration of sludge:25 mg dry matter/L
- Initial cell/biomass concentration: 4.68 g suspended solids/L
- Water filtered: no - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 31 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 19.4 – 21.1 °C without direct lighting
- pH: at test start not reported; pH at the end of the test and before addition of HCl: 7.5 in the test vessel with test item
- pH adjusted: no
- Aeration of dilution water: The culture flasks were filled with medium and inoculum and aerated for 72 h with purified, CO2-free, moistened air to purge the system of CO2
- Suspended solids concentration: 25 mg dry matter/L
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: culture flasks
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: The culture flasks were filled with medium and inoculum and aerated for 72 h with purified, CO2-free, moistened air to purge the system of CO2
- Measuring equipment: Carbon Analyser TOC multi N/C 2100S, Analytik Jena
- Test performed in closed vessels: yes
- Details of trap for CO2: The emitted CO2 was trapped in 0.25 m NaOH. Two scrubber flasks containing 100 mL each were connected in series to the test vessels. The initial inorganic carbon value of the 0.25 m NaOH was separately determined in each flask
SAMPLING
- Sampling frequency: sampling was performed on days 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29. On day 28, 5 mL HCl were added to each test flask to drive off dissolved CO2.
- Sampling method: During the test, 10 samples (1 mL each) from each front scrubber flask were taken to determine CO2 evolution.
- Sample storage before analysis: no
CONTROL AND BLANK SYSTEM
- Inoculum blank: performed
- Abiotic sterile control: performed
- Toxicity control: performed
- Other: aniline was used as readily biodegradable positive control
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 40
- Sampling time:
- 28 d
- Details on results:
- 10-day-window: day 8 – 28
degradation at the end of 10-day-window 37 %
degradation at the end of the test 40 %
BOD5 / COD results
- Results with reference substance:
- The reference substance aniline reached the pass level of >60% degradation within 7 d.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Stearyl sulfobetaine proved to be not readily biodegradable (40% biodegradation after 28 d).
- Executive summary:
The ready biodegradation of the stearyl sulfobetaine was investigated in a study conducted according to OECD Guideline 301B (Ready Biodegradability: CO2 Evolution Test; 1992) and EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test; 2008) over a period of 28 days and using non-adapted activated sludge as inoculum. The biodegradation rate was determined by measurement of CO2 evolution. Inoculum blank, procedural/functional control with reference substance aniline, and toxicity control using 31.1 mg/L test item and 27.6 mg/L reference compound (ca. 20 mg organic carbon/L each) were performed.
This study is regarded as reliable without restriction and satisfies the guideline requirements for ready biodegradation.
The test substance proved to be not readily biodegradable under the test conditions employed (40 % biodegradation after 28 d). The functional control reached the pass level > 60% after 14 d. In the toxicity control containing both test and reference item 60.3% biodegradation occurred within 14 d thus indicating that the test item was not inhibitory at the concentration tested.
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