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Diss Factsheets
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EC number: 953-863-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Oct. 12-Nov. 30, 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Distillate of tobacco leaf, Nicotiana tabacum, solanaceae
- EC Number:
- 953-863-9
- IUPAC Name:
- Distillate of tobacco leaf, Nicotiana tabacum, solanaceae
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations
- Sampling method: before and after media renewal, test end
- Sample storage conditions before analysis: If not measured on day of sampling, samples were stored at -18 degrees C.
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 99.3 uL of test item was transferred to a glass flask and filled with 1000 mL dilution water. This was stirred for 15-30 minutes.
- Other relevant information: Cu-reduced dilution water enriched with CaCl2.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Age at study initiation (mean and range, SD): 4-24 hrs old
- Source: German Federal Environment Agency, Institut fur Wasser-, Boden-, und Lufthygiene
- Age of parental stock (mean and range, SD): At least 3 weeks
- Feeding during test: No
Study design
- Test type:
- semi-static
- Water media type:
- other: Cu-reduced dilution water enriched with CaCl2.
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250-350 mg CaCO3/L
- Test temperature:
- 19.0-19.5 degrees C
- pH:
- 7.78-8.31
- Dissolved oxygen:
- 7.88-8.85 mg/L
- Nominal and measured concentrations:
- Nominal: 6.25, 12.5, 25.0, 50.0, and 100 mg/L
Active ingredient (nicotine): 1.39, 2.78, 5.55, 11.1, and 22.2 mg/L
Geometric mean measured: 0.944, 1.91, 3.71, 7.49, and 15.1 mg a.s./L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open, but covered with glass
- Material, size, headspace, fill volume: 50 mL glass beakers
- Volume of solution: 50 mL
- Aeration: yes, open to atmosphere
- Renewal rate of test solution (frequency/flow rate): Renewed at 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 0.929 mg/L
- Metals: < 4.053 ug/L
- Chlorine: <0.02 mg/L
- Alkalinity: 1.8 mmol/L
- Ca/mg ratio: 0.9/0.3
- Conductivity: 283 uS/cm
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: 698-779 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mobility, appearance and behaviour, daily
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10, and 100 mg test item/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Sensitivity of test organisms is tested twice per year using K2Cr2O7.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.91 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.84 mg/L
- 95% CI:
- 2.79 - 5.3
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: yes, daphnids at the 1.91 mg a.s./L concentration were less active than animals in the 0.944 mg a.s./L and control concentrations at 24 and 48 hrs. At higher concentrations, living daphnids were observed on the ground with little movement and twitching.
Any other information on results incl. tables
One daphnid was immobilized in the test medium control.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hr EC50 for Daphnia magna is 3.84 mg/L.
- Executive summary:
The toxicity of the dossier substance was determined in an OECD guideline 202 test. Concentrations of 0, 6.25, 12.5, 25.0, 50.0, and 100 mg/L (nominal) were tested. Concentrations of the nicotine in the test substance were measured giving the following concentrations based on the geometric mean 0.944, 1.91, 3.71, 7.49, and 15.1 mg a.s./L. The 48-hr EC50 for Daphnia magna is 3.84 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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