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EC number: 486-070-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 20 AUG 2007 to 3 DEC 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- No analytical monitoring
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Based on the information provided by the Sponsor, the test item is soluble in water at a concentration >1 g/L. Therefore, 502.17 mg of the test item was dissolved in 284 mL tap water by ultrasonic treatment for five minutes and intense stirring for 15 minutes at room temperature. In this way a clear solution was obtained. Then, 16 mL synthetic wastewater and 200 mL activated sludge inoculum were added.
- Differential loadings: No (limit test)
- Controls: two controls containing only tap water, synthetic wastewater and inoculum were tested in parallel to the single test concentration of the test item under identical test conditions.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no, aerobic activated sludge came from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf, CH) treating predominantly domestic wastewater.
- Method of cultivation: During the holding period of two days prior to use, the sludge was fed daily with 50 mL synthetic wastewater* per liter and was kept at room temperature under continuous aeration until use. The pH of the activated sludge inoculum was 6.5.
- Preparation of inoculum for exposure: The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. An aliquot of washed sludge was suspended in tap water to obtain a concentration equivalent to 3 g dry material per liter.
- Pretreatment: no
- Initial biomass concentration: 3 g dry material per liter
* Synthetic sewage feed:
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2 × 2H2O
0.2 g MgSO4 × 7H2O
2.8 g K2HPO4
filled up to a final volume of 1 liter with deionized water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- No data
- Test temperature:
- The temperature measured in test media in one control was 20°C at the start and at the end of the incubation period
- pH:
- 7.3 - 7.9 (test item)
7.2 - 8.1 (reference item) - Dissolved oxygen:
- 8.1 - 8.8 mg/L (test item)
8.5 - 8.9 mg/L (reference item)
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal concentration: 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, headspace, fill volume: The test was performed in 2000-mL glass beakers, filled with a 500-ml mixture containing synthetic wastewater, test medium and activated sludge.
- Aeration: During the incubation period of exactly 3 hours, all test media and the controls were continuously aerated by intense stirring on magnetic stirrers, to avoid possible foaming and/or stripping of the test item.
- No. of organisms per vessel: not applicable
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Biomass loading rate: sludge concentration = The inoculum had a sludge concentration of 2.2 g/L dry weight (corresponding to about 0.9 g dry material per liter test medium)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon, Particulate matter, Metals, Pesticides, Chlorine, Alkalinity, Ca/mg ratio, Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement:The pH values and dissolved oxygen concentrations were determined after the addition of synthetic wastewater and activated sludge in all test media and the controls at the start and at the end of the 3-hour incubation period. The water temperature was measured in one control at the start and at the end of the incubation period. Before the addition of activated sludge and synthetic wastewater, the appearance of the test media was recorded.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Lighting: no data
EFFECT PARAMETERS MEASURED : For measurement of the respiration rate a well-mixed sample of each test medium was poured into a BOD-flask after three hours incubation time, and was not further aerated. Then the dissolved oxygen concentration was measured with an oxygen electrode (WTW TriOxmatic® 300 and an oxygen meter WTW Oxi 539, Wissenschaftlich-Technische Werkstaetten WTW, Weilheim/Germany), and was continuously recorded. During measurement, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption rate (in mg O2 L-1 minute-1) was determined from the linear part of the respiration curve in the range 6.5–2.5 mg O2/L. In case of very rapid oxygen consumption, the range used was below the limits indicated above but always within the linear part of the respiration curve. In case of low oxygen consumption the rate was determined over a period of at least ten minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Justification for using less concentrations than requested by guideline: a limit test has been performed to demonstrate that the test item has no toxic effect on activated sludge up to the nominal concentration of 1000 mg/L.
- Range finding study: none
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol tested at 5, 16 and 50 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test item Dimethyl 2-Methyl Glutarate had no significant inhibitory effect (<15%) on the respiration rate of activated sludge after the incubation period of 3 hours at the limit test concentration of 1000 mg/L.
Thus, the 3-hour NOEC (EC15) of Dimethyl 2-Methyl Glutarate to activated sludge microorganisms was at least 1000 mg/L. This value might even be higher but concentrations above 1000 mg/L were not tested. The 3-hour EC20, EC50, and EC80 could not be calculated but were clearly higher than 1000 mg/L. - Results with reference substance (positive control):
- The 3-hour EC50 of the reference item 3,5-dichlorophenol (positive control) was calculated to be 14 mg/L (the 95% confidence limits could not be calculated). The 3-hour EC50 is within the guideline-recommended range of 5–30 mg/L, confirming suitability of the activated sludge used.
- Reported statistics and error estimates:
- The 3h-EC50, -EC20 and -EC80 of the test item and their 95% confidence limits could not be calculated due to the absence of a toxic effect. For the reference substance, the probit analysis was used.
- Validity criteria fulfilled:
- yes
- Remarks:
- The EC50 of the reference substance is in the acceptance range and the oxygen consumption rates of the 2 controls differed by less than 15% (3%).
- Conclusions:
- Dimethyl 2-methyl glutarate has no toxic effect on the respiration rate of activated sludge microorganisms at the limit test concentration of 1000 mg/L.
- Executive summary:
The inhibitory effect of DIMETHYL 2-METHYL GLUTARATE on the respiration rate of aerobic wastewater microorganisms of activated sludge was investigated in a 3-hour respiration inhibition test according to the EU Commission Directive 88/302/EEC, Part C.11, and the OECD Guideline for Testing of Chemicals, No. 209.
A limit test was performed with one concentration of DIMETHYL 2-METHYL GLUTARATE of nominal 1000 mg/L.
In addition, two controls and three different concentrations of the reference item 3,5 dichlorophenol (5, 16, and 50 mg/L) were tested in parallel. After a 3 -hour incubation time, the inhibition of the respiration rates were investigated via a determination of oxygen consumption rates.
The results of the reference treatments (EC50 = 14 mg/L within the guideline-recommended range of 5 - 30 mg/L) confirmed suitability of the activated sludge and the method used.
The test item DIMETHYL 2-METHYL GLUTARATE had no significant inhibitory effect on the respiration rate of activated sludge after the incubation period of three hours at the limit test concentration of 1000 mg/L. Thus, the 3-hour NOEC of DIMETHYL 2-METHYL GLUTARATE to activated sludge microorganisms was > = 1000 mg/L. The 3-hour EC20, EC50 and EC80 were clearly higher than 1000 mg/L.
Reference
Table 1 : Influence of Dimethyl 2 -methyl glutarate and 3, 5-dichlorophenol on the oxygen consumption of activated sludge
Vessel No. |
Test chemical |
Nominal concentration of test chemical (mg/L) |
Oxygen consumption rate (mg O2/L min-1) |
Inhibition (%) |
A B |
Control Control |
0 0 |
1.033 1.062 |
|
Mean Deviation (%) |
|
|
1.047 2.8 |
|
1 2 3 5 |
3,5-dichlorophenol 3,5-dichlorophenol 3,5-dichlorophenol Dimethyl 2 -methyl glutarate |
5 16 50 1000 |
0.937 0.354 0.105 0.975 |
10.6 66.2 90.0 6.9 |
-% inhibition: increased oxygen consumption rate relative to control
Description of key information
The 3-hour NOEC of dimethyl 2-methyl glutarate to activated sludge microorganisms was > = 1000 mg/L. The 3-hour EC20, EC50 and EC80 were clearly higher than 1000 mg/L. Hence, dimethyl 2-methyl glutarate has no toxic effect on the respiration rate of activated sludge microorganisms.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
One experimental study, scored as Klimisch 1, is available (Seyfried B., 2007) and selected as a key study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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