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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl octadecanoate, chloro
- Molecular formula:
- C17H30Cl4COOCH3, C17H29Cl5COOCH3 , and C17H28Cl6COOCH3 (primary isomers based on 35-40% Cl by weight)
- IUPAC Name:
- Methyl octadecanoate, chloro
- Reference substance name:
- Methyl hexadecanoate, chloro
- Molecular formula:
- C15H28Cl4COOCH3 and C15H27Cl5COOCH3 (primary isomers based on 35-40% Cl by weight)
- IUPAC Name:
- Methyl hexadecanoate, chloro
- Reference substance name:
- Methyl dodecanoate, chloro
- Molecular formula:
- C19H31Cl5COOCH3 and C19H30Cl6COOCH3 (primary isomers based on 35-40% Cl by weight)
- IUPAC Name:
- Methyl dodecanoate, chloro
- Reference substance name:
- Soybean oil, epoxidized
- EC Number:
- 232-391-0
- EC Name:
- Soybean oil, epoxidized
- Cas Number:
- 8013-07-8
- Molecular formula:
- C57H98O12
- IUPAC Name:
- Epoxidized Soybean Oil
- Test material form:
- liquid
- Details on test material:
- A complex combination of chlorinated fatty acid mtethyl esters that are predominantly C18 (~ 70 – 85%) and C16 (~ 15 - 30%) in length. The chlorination levels by weight are between 35-40%. The chlorination process is largely random, though chlorination will occur first by direct addition at unsaturated sites in the unsaturated methyl esters present in the starting material.
Constituent 1
Constituent 2
Constituent 3
additive 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- Before conducting the preliminary range finding study, Doverguard E35 was checked for solubility. Doverguard E35 formed emulsion in test media (OECD media) at the concentration of 200 mg/mL. Further, solubility was checked in acetone, dimethyl sulfoxide (DMSO) and dimethylformamide (DMF). In acetone, test item was observed soluble at the concentration level of 1000 mg/mL. This stock solution was serially diluted to prepare the concentration levels of 500, 250, 125, 62.5, and 31.25 mg/mL. To check the precipitation, these stock solutions were added at the concentration of 0.1 mL/L in test media. Precipitation was observed at 100, 50, 25, 12.5 mg/L. Visually clear solution was observed but very tiny droplets were observed under microscope at 6.25 mg/L. No precipitation was observed at 3.125 mg/L. A test solution of 6.25 mg/L for the study was selected as the upper limit.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain: ATCC 22662
Source: American Type Culture Collection, Manassas, USA
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- samples were evaluated up to 96 hours
Test conditions
- Test temperature:
- 22 to 23 °C
- pH:
- 7.49 to 8.12
- Nominal and measured concentrations:
- 0.0 (control), 0.0 (vehicle control), and 6.25 mg/L
- Details on test conditions:
- Initial Mean Number of Cells/mL: 5292
- Reference substance (positive control):
- no
- Remarks:
- solvent control
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- not determinable
- Remarks:
- No effects observed.
- Details on results:
- There was no effect on algal growth by either the test material or the solvent (acetone) at the upper limit of 6.25 mg/L, which was the maximum concentration that could be tested just prior to the onset of significant test material precipitation.
Applicant's summary and conclusion
- Conclusions:
- C16 and C18 chlorinated fatty acid methyl esters do not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to 6.25 mg/L, the maximum concentration that could be tested.The EC50, NOEC and LOEC value were greater than 6.25 mg/L
- Executive summary:
This study was performed to assess effects of C16 and C18 chlorinated fatty acid methyl esters on the growth of the alga Pseudokirchneriella subcapitata,caused by exposure under static conditions.
Exponentially growing cultures of P. subcapitata were exposed to the nominal test concentrations of 0.0 (control), 0.0 (vehicle control), and 6.25 mg/L. The highest test concentration was selected based on the solubility, precipitation and result of range finding study. Algal cultures were assessed for their growth by visual cell count at 24, 48, 72, and 96 h.
There was no significant alteration observed in the algal growth (biomass), yield, and growth rate of the group exposed to the test material when compared with that of the vehicle control group. C16 and C18 chlorinated fatty acid methyl esters do not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to 6.25 mg/L.The EC50, NOEC and LOEC value were greater than 6.25 mg/L.
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