4-(4-chlorophenyl)-2-phenyl-2-[(1H-1,2,4-triazol-1-yl)methyl]butanenitrile

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-11-01 to 1989-11-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Remarks:

Closed bottle test according to OECD Guideline No. 301D and EEC Directive 67/548 Annex V C.6 as published in 84/449/EEC.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

4-(4-chlorophenyl)-2-phenyl-2-[(1H-1,2,4-triazol-1-yl)methyl]butanenitrile

EC Number:

406-140-2

EC Name:

4-(4-chlorophenyl)-2-phenyl-2-[(1H-1,2,4-triazol-1-yl)methyl]butanenitrile

Cas Number:

114369-43-6

Molecular formula:

C19H17ClN4

IUPAC Name:

4-(4-chlorophenyl)-2-phenyl-2-[(1H-1,2,4-triazol-1-yl)methyl]butanenitrile

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Temperature 20 ± 1 °C

The test substance was dissolved in chloroform (Fisons A.R. Grade) to give
a stock solution of 56 mg/10 mL. Six 10 µL aliquots of stock solution were
placed on Whatman GFA paper and the solvent allowed to evaporate to
dryness. One piece of paper was placed in each test bottle prior to filling
with inoculated medium. Filter paper blanks were prepared in the same manner
using solvent only.

Results and discussion

Preliminary study:

The test substance attained only 17% biodegradation after 28 days

and cannot be termed as readily biodegradable.

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

ca. 17

Sampling time:

28 d

BOD5 / COD results

BOD5 / COD

Key result

Parameter:

COD

Value:

3.72 other: mg O2/L

Results with reference substance:

Sodium benzoate attained 93 - 99% biodegradation within 28 days.
Aniline attained 65% biodegradation within 28 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Assessment of ready biodegradability, closed bottle test according to OECD Guideline No. 301, with a 28 day duration. Percentage Biodegradation after 28 days was 17%, therefore determined to not be readily biodegradable. 

Executive summary:

Dissolved oxygen measurements for the test substance and standard
substance solutions, together with the inoculated and non-inoculated blank controls, at 0, 5, 15 and 28 days. 
The test substance attained only 17% biodegradation after 28 days and
cannot, therefore, be termed as readily biodegradable.
Sodium benzoate attained 93 - 99% biodegradation within 28 days.
Aniline attained 65% biodegradation within 28 days.