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EC number: 421-300-1 | CAS number: 138564-59-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-03-27 to 1996-03-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 421-300-1
- EC Name:
- -
- Cas Number:
- 138564-59-7
- Molecular formula:
- C12H9N3O2S
- IUPAC Name:
- 5-methyl-2-[(2-nitrophenyl)amino]thiophene-3-carbonitrile
- Test material form:
- solid: particulate/powder
- Details on test material:
- 97.7%
Item code QA405K
Lilly lot no: 356MO1
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor and 356M01
- Purity test date: 1995-11-10
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature in a tightly secured container.
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolved in acetone to act as the carrier in the stock solution to disperse the test substance in water.
FORM AS APPLIED IN THE TEST (if different from that of starting material)
0 (water and acetone controls), 125, 250, 500, 800, and 1500 µg test ingredient/L
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Actual intennediate 290437 concentrations were determined at test initiation and termination. At test initiation, 7 .5 ml was collected from each solution prior to distribution to the replicates. At test termination, a composite sample of 12 ml (3 ml from each of the four vessels containing organisms) was collected from the four replicates containing animals at each concentration. All samples were collected into appropriately labeled containers, diluted with methanol (to a final methanol concentration of 25%), and immediately submitted for analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dissolution of the test substance into a stock solution of acetone, and then diluting this in water to the proper test concentrations.
- Controls: Two vehicle controls (acetone/water mixture and just water) were run simultaneously with the main test
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone acting as a dispersant and water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 250 ul acetone/L of dilution water
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Strain/clone: Daphnia magna
- Source: Lilly Environmental Toxicology Laboratory
- Age of parental stock (mean and range, SD): All were 24 hours old or younger
- Feeding during test: Daphnids were not fed during the test, but were prior to testing.
ACCLIMATION
- Acclimation period: Less than or equal to 24 hours
- Acclimation conditions (same as test or not): same as test
- Food type: unicellular green algae and cerophyl
- Frequency: daily
- Health during acclimation (any mortality observed): none
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
A plastic pipet (4-mm I.D.) was used to place five first-instar daphnids into each of the 4 replicates/treatment.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
Test conditions
- Hardness:
- 120 mg/L as CaCO3
- Test temperature:
- 19.7 +-0.2 degrees C
- pH:
- 8.1-8.2
- Dissolved oxygen:
- 9.4 +- 0.3 mg/L
- Salinity:
- 121 mg/L as CaCO3
- Conductivity:
- 211 umhos
- Nominal and measured concentrations:
- The average assayed intermediate 290437 concentrations for the study were 108, 228, 448, 730, and 1312 ug test ingredient/L at nominal concentrations of 125, 250, 500, 800, and 1500 ug test ingredient/L, respectively
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Borosilicate glass, 250 ml, 50 ml, 200 ml
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Fresh condition well water 7 times/week
- Alkalinity: 115 mg/L as CaCO3
- Conductivity: 244 umhos
- Culture medium different from test medium: Same as test medium
- Intervals of water quality measurement: 7 times/week, at test intiation, and 24/48 hours into the test.
OTHER TEST CONDITIONS
- Adjustment of pH: Adjusted to 8.1
- Photoperiod: 16 light / 8 dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Physical condition of each organism at intiation, 24 h, and 48 h
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 274 - <= 370 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 108 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- - Behavioural abnormalities:In the water control, on organism was hypoactive at 24 hours; additionally, at 48 hours one organism was hypoactive, one was prostrate, and one was immobile. In the solvent control a single organism was hypoactive at 24 hours and prostrate at 48 hours. No treatment-related immobilization or other physical signs of toxicity were observed in Daphnia magna exposed to an average assayed test substance concentration of 108 ug/L. At average assayed test substance concentrations >227 ug/L, hypoactivity, prostration, and immobilization were observed.
- Mortality of control: None
- Other adverse effects control: In the water control, on organism was hypoactive at 24 hours; additionally, at 48 hours one organism was hypoactive, one was prostrate, and one was immobile. In the solvent control a single organism was hypoactive at 24 hours and prostrate at 48 hours. - Reported statistics and error estimates:
- Statistical Evaluation
The EC50 value was calculated by probit analysis (SAS, 1990). All values expressed as "x ± y" represent means± standard deviations and were calculated using SAS (1990) or a hand calculator.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study validly assessed the test substance's short-term toxicity to aquatic invertebrates according to the specified guidelines.
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