Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 612-154-1 | CAS number: 6147-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 March - 15 April 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- The temperature of the tests remained within 20 °C 2 °C but a few vessels did not remain within a constant 1 °C. This has not thought to have had a significant impact on the overall test result as the control vessels met the validity criteria.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- 66% purity
Aqueous solution - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution Series - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: The ephippia were incubated no more than 96h prior to test commencement, therefore the neonates were less than 24h old at test commencement
- Source: The test organisms were sourced from ephippia supplied by Cheshire Eco Solutions Ltd, from MicroBioTests
- Feeding during test : The animals were fed Pseudokirchneriella subcapitata during incubation and not fed during the test.
ACCLIMATION
- Acclimation period: The ephippia were incubated no more than 96h prior to test commencement
- Type of food: Pseudokirchneriella subcapitata - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Observations were recorded at 24 hours and 48 hours of exposure to test substance
- Hardness:
- Not specified
- Test temperature:
- 20 °C 2 °C. Constant within 1 °C
- pH:
- 6-9
- Dissolved oxygen:
- >3 mg/l
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 10, 32, 100, 320, 1000, 3200,10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass crystallising dishes
- Material, size, headspace, fill volume: 100 ml capacity, borosilicate
- Volume of solution: 50ml
- Aeration: covered with soda glass watch covers
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2 vessels per concentration
- No. of vessels per control (replicates): 2 vessels per concentration
- No. of vessels per vehicle control (replicates): 2 vessels per concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO reconstituted water (as specified in OECD 202)
- Intervals of water quality measurement: Carried out at each concentration at 0h and 48h
EFFECT PARAMETERS MEASURED: Immobilisation
OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark
RANGE-FINDING STUDY
- Test concentrations: 1000, 100, 10 and 1 mg/l
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 304 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- ca. 1 730 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
none reported
Observations on body length and weight: None reported
- Mortality of control: 5%
- Abnormal responses: None reported - Results with reference substance (positive control):
- - Results with reference substance valid?
YES
- Relevant effect levels: yes
- Limit test: no
- Dose-response test: yes
- EC50 24h: 0.6 - 2.1 mg/L - Reported statistics and error estimates:
- Results for both range-finding and definitive tests were calculated by Linear Interpolation within the CETIS suite of statistical analysis.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°
1272/2008. - Executive summary:
The short-term toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater crustacean Daphnia magna was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.
A range-finding test was conducted at concentrations of 1000, 100, 10 and 1 mg/l, plus four control vessels to determine the concentrations to be used during the definitive test.
In the definitive test, concentrations of 10, 32, 100, 320, 1000, 3200,10000 mg/L, plus four control vessels were used.
The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L.
Reference
Nominal Concentration (mg/l) | Number immobile at 24h | Number immobile at test termination (48h) | Total Exposed | ||
Replicate A | Replicate B | Replicate A | Replicate B | ||
10 | 0 | 0 | 0 | 1 | 20 |
32 | 0 | 0 | 1 | 0 | 20 |
100 | 1 | 0 | 2 | 2 | 20 |
320 | 2 | 1 | 5 | 6 | 20 |
1000 | 3 | 2 | 9 | 9 | 20 |
3200 | 10 | 9 | 10 | 9 | 19 |
10000 | 11 | 10 | 11 | 10 | 21 |
Description of key information
The short-term toxicity of 2-propen-1-amine, N-2-propen-1-yl, hydrochloride to the freshwater crustacean Daphnia magna was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.
A range-finding test was conducted at concentrations of 1000, 100, 10 and 1 mg/l, plus four control vessels to determine the concentrations to be used during the definitive test.
In the definitive test, concentrations of 10, 32, 100, 320, 1000, 3200,10000 mg/L, plus four control vessels were used.
The 48-hour EC50 was 304 mg/L and the 48-hour NOEC 32 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 304 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.