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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 19 April 2021 to 21 April 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 April 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Batch/Lot number: 381890
Purity: 99.8% - Analytical monitoring:
- yes
- Details on sampling:
- samples were taken and analyzed at the beginning and at the end of the experiment.
- Vehicle:
- yes
- Remarks:
- Reconstituted water
- Details on test solutions:
- A stock solution with a nominal concentration of 100.0 mg/L (nominal) was prepared with direct addition of the test item and mixed into the test medium (ISO Medium).
The test solution was prepared just before introduction of the Daphnia (start of the experiment). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: (Straus)
- Justification for species other than prescribed by test guideline: Daphnia magna is the standard species of the acute immobilisation test.
- Age at study initiation (mean and range, SD): Less than 24 h old at the beginning of the test.
- Source: Hungarian University of Agriculture and Life Sciences, Department of Aquaculture 2100 Gödöllő, Páter Károly u. 1. – Hungary
- Feeding during test: not fed during the test
ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: The Daphnia were fed with concentrated algal suspension of Raphidocelis subcapitata during the holding. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 250 mg/L (as CaCO3)
- pH:
- in the range of 7.85–7.95
- Dissolved oxygen:
- in the range of 8.2 – 8.7 mg/L
- Nominal and measured concentrations:
- 100.0 mg/L (nominal);
Measured concentration: 98.8 mg/L at the start and 97.1 mg/L at the end - Details on test conditions:
- TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): open
- Volume of solution: at least 5 mL test solution/animal
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observation of environmental parameters:
The water temperature was determined daily in one control test vessel and continuously with a min/
max thermometer in the climate chamber.
The oxygen concentration and pH were measured in both the control and the test concentration level
(in one test vessel) at the start and at the end of the experiment.
Observation of animals:
The number of mobile and immobilized test animals was observed and recorded 24 and 48 hours af
ter the start of the test. Those animals not able to swim within 15 seconds after gentle agitation of the
test beaker were considered to be immobile.
RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1, 1, 10, 100.0 mg/L
- Results used to determine the conditions for the definitive study: no significant inhibition was obs
erved at any concentration during the preliminary range-finding test - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The number of immobilised animals and the percentage of immobility were determined at the 24th
and 48th hour (1 animal at 48 hours, 5%).
In addition to immobility, no abnormal behaviour or appearance of test animals was detected. - Results with reference substance (positive control):
- The 24h EC50: 0.73 mg/L, (95 % confidence limits: 0.69 – 0.78 mg/L)
This result falls within the acceptable range (0.6 mg/L to 2.1 mg/L) hence this reference study shows
that the acute immobilization test on Daphnia magna is valid in this laboratory. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.
- Executive summary:
Acute toxicity of the test item on Daphnia magna was assessed in an acute immobilisation test, over an
exposure period of 48 hours in a static test system, according to OECD Guideline 202 under GLP.
The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.
Reference
Description of key information
Acute toxicity of the test item on Daphnia magna was assessed in an acute immobilisation test, over an exposure period of 48 hours in a static test system, according to OECD Guideline 202 under GLP.
The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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